| 稳定过表达p50基因细胞株的构建及鉴定 |
| |
| 引用本文： | 陈炜,高娜,王嘉丽,张俊磊,田衍平,安静. 稳定过表达p50基因细胞株的构建及鉴定[J]. 局解手术学杂志, 2009, 18(2) |
| |
| 作者姓名： | 陈炜 高娜 王嘉丽 张俊磊 田衍平 安静 |
| |
| 作者单位： | 陈炜,王嘉丽,张俊磊,CHEN Wei,WANG Jia-li,ZHANG Jun-lei(第三军医大学基础医学部微生物学教研室,重庆市微生物工程实验室,重庆,400038);高娜,田衍平,GAO Na,TIAN Yan-ping(第三军医大学基础医学部组织胚胎学教研室,重庆,400038);安静,AN Jing(首都医科大学基础医学院微生物学教研室,北京,100069) |
| |
| 摘 要： | 目的 筛选出稳定过表达p50基因的细胞克隆株ECV-p50,为研究dynei-n-dynactin复合体在登革病毒逆行性运输中的作用奠定基础.方法 将构建的pRe-p50质粒稳定转染至ECV304细胞,通过间接免疫荧光染色和免疫印迹实验进行鉴定.结果 经间接免疫荧光染色和免疫印迹实验验证,稳定过表达p50基因的细胞克隆株命名为ECV-p50.结论 成功建立了稳定过表达p50基因的细胞克隆株ECV-p50,为后续研究积累了有价值的实验资料.
|
| 关 键 词： | 病毒 微管骨架 逆行性运输 过表达 |
Construction and appreciation of p50 gene cells stable overexpression |
| |
| CHEN Wei,GAO Na,WANG Jia-li,ZHANG Jun-lei,TIAN Yan-ping,AN Jing. Construction and appreciation of p50 gene cells stable overexpression[J]. Journal of Regional Anatomy and Operative Surgery, 2009, 18(2) |
| |
| Authors: | CHEN Wei GAO Na WANG Jia-li ZHANG Jun-lei TIAN Yan-ping AN Jing |
| |
| Affiliation: | Department of Microbiology;College of Basic Medicine;Third Military Medical University;Chongqing 400038;China |
| |
| Abstract: | Objective To construct stable p50 gene overexpression cells for studying the role of dynein-dynactin in the retrograde transport of Dengue virus.Methods ECV304 cells were stable transfected by pRe-p50.Then the stable p50 gene overexpression cells were confirmed by western blot and indirect immunofluorescence assay.Results The stable p50 gene overexpression cells were selected and identified,named ECV-p50.Conclusion The stable p50 gene overexpression cells have been constructed successful,which lays a base f... |
| |
| Keywords: | p50 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
