盐酸多奈哌齐对D-半乳糖致老年性痴呆大鼠治疗作用的机制

目的：从自由基方面探讨盐酸多奈哌齐对D-半乳糖致老年性痴呆大鼠治疗作用的机制。方法：雄性Wistar大鼠36只，采用区组随机分组法按体重随机分为正常对照组、模型组及盐酸多奈哌齐组，每组12只。腹腔注射D-半乳糖7周，制作大鼠老年痴呆模型，通过Morris水迷宫实验、跳台实验及皮层和海马组织病理检查，观察盐酸多奈哌齐对老年性痴呆的影响，并测定脑内的脂质过氧化物（MDA）、超氧化物歧化酶（SOD）。结果：与模型组比，盐酸多奈哌齐组大鼠水迷宫第4天及第5天达到平台的潜伏期及游程缩短（P<0.05或P<0.01），第4天及第5天向角变小（P<0.05），第1天、第2天跳台的错误次数减少（P<0.05），脑组织MDA降低（P<0.05），SOD升高（P<0.05）。盐酸多奈哌齐组大鼠皮层及海马神经细胞排列较整齐，变性、坏死的神经细胞及嗜神经细胞现象少于模型组。结论：盐酸多奈哌齐对D-半乳糖致老年性痴呆大鼠有治疗作用，其机制除抑制乙酰胆碱酯酶以外，与其减少自由基损伤有关。

Therapeutic effect of Donepezil hydrochloride on Alzheimer''''s disease rats

Objective To explore the mechanism of therapeutic effect of Donepezil hydrochloride on Alzheimer's disease(AD) rats.Methods According to weight,36 rats were divided into normal group,model group and Donepezil hydrochloride group.AD rat model was set up by injecting D-galactose into abdominal cavity for seven weeks,learning and memory function of rats was determined by using Morris water maze and Step-down test.The section of rat cerebral cortex and hippocampus were stained with haematoxylin eosin(HE),and the effect of Donepezil hydrochloride was observed by detecting the MDA content and SOD activity in cerebral tissue.Results Compared with model group,latency and distance of Donepezil hydrochloride rats shortened on the fourth day and the fifth day,starting angle of Donepezil hydrochloride rats shortened on the fourth day and the fifth day in the Morris water maze test,error times of Donepezil hydrochloride rats decreased on the first day and the second day in Step-down test;MDA content in cerebral tissue of Donepezil hydrochloride rat was deceased(P<0.05),SOD activity in cerebral tissue of Donepezil hydrochloride rats was increased(P<0.05),Donepezil hydrochloride significantly reduced the pathological changes of AD rats.Conclusion Donepezil hydrochloride has theraputic effect on AD rats by decreasing free radical injury.