| 羊膜对人脐血干细胞分化为多巴胺能神经元的作用 |
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| 引用本文： | 李荣平,季凤清,孙海梅,王丹妮,曾小蓓,赵春礼,杨慧. 羊膜对人脐血干细胞分化为多巴胺能神经元的作用[J]. 解剖学报, 2008, 39(6): 790-794 |
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| 作者姓名： | 李荣平 季凤清 孙海梅 王丹妮 曾小蓓 赵春礼 杨慧 |
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| 作者单位： | 首都医科大学组织学胚胎学教研室;首都医科大学神经科学研究所,北京,100069 |
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| 基金项目： | 国家重点基础研究发展规划(973计划) ， 北京市教委科技发展计划 |
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| 摘 要： | 目的 探讨羊膜上皮细胞对人脐血干细胞定向分化为多巴胺能神经元的作用.方法 体外分离、原代培养人羊膜上皮细胞,通过高速离心制备成条件培养液(CM)对P1代脐血十细胞进行诱导,倒置相差显微镜观察细胞形念的变化,应用免疫荧光染色和免疫印迹法检测其酪氨酸羟化酶(TH)及多巴胺转运体(DAT)蛋白的表达情况.结果 P1代脐血干细胞经羊膜上皮细胞条件培养液诱导后,TH及DAT阳性细胞率明显高于对照组,与对照组比较有显著性差异(P<0.01),免疫印迹分析结果 与免疫荧光染色结果 一致.结论 羊膜上皮细胞能诱导人脐血问充质干细胞定向分化为多巴胺能神经元.
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| 关 键 词： | 干细胞 诱导分化 多巴胺能神经元 羊膜上皮细胞 免疫组织化学 人脐带血 |
| 收稿时间： | 2007-07-31 |
| 修稿时间： | 2007-12-03 |
THE FUNCTION OF AMNION IN TNE DIFFERENTIATION OF HUMAN UMBILICAL CORD BLOOD STEM CELLS INTO DOPAMINERGIC NEURONS |
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| LI Rong-ping,JI Feng-qing,SUN Hai-mei,WANG Dan-ni,ZENG Xiao-bei,ZHAO Chun-li,YANG Hui. THE FUNCTION OF AMNION IN TNE DIFFERENTIATION OF HUMAN UMBILICAL CORD BLOOD STEM CELLS INTO DOPAMINERGIC NEURONS[J]. Acta Anatomica Sinica, 2008, 39(6): 790-794 |
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| Authors: | LI Rong-ping JI Feng-qing SUN Hai-mei WANG Dan-ni ZENG Xiao-bei ZHAO Chun-li YANG Hui |
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| Affiliation: | 1. The Department of Histology and Embryology; 2. Beijing Institute for Neuroscience, Capital University of Medical Science, Beijing 100069, China |
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| Abstract: | Objective To investigate the function of amnion endothelial cell in the differentiation of human umbilical cord blood stem cells into dopaminergic neurons.Methods Primary human amnion endothelial cells were separated and cultured in vitro;the conditioned medium(CM) was prepared through high speed centrifugation.The cord blood mesenchymal stem cells of P_1 passage were induced by the conditioned medium,and the mophology of cells was observed under the inverted phase contrast microscope.The expression of tyro... |
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| Keywords: | Stem cells Inducing Differentiation Dopaminergic neurons Amnion endothelial cells Immunohistochemistry Human umbilical cord blood |
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