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Curcumin protects against staurosporine toxicity in rat neurons |
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| Authors: | Xiao-Yan Qin Ji-Hui Lv Jia Cui Xue Fang Yan Zhang |
| Institution: | 1. State Key Laboratory of Biomembrane and Membrane Biotechnolog, College of Life Sciences, Peking University, Beijing, 100871, China 3. Laboratory of Biotechnology and State Key Laboratory of Chinese Ethnic Minority Traditional Medicine, College of Life & Environmental Science, Minzu University of China, Beijing, 100081, China 2. Beijing Geriatric Hospital, Beijing, 100095, China |
| Abstract: | ObjectiveCurcumin is extracted from the turmeric plant (Curcuma longa Linn.) and is widely used as a food additive and traditional medicine. The present study investigated the activity of curcumin against staurosporine (STS) toxicity in cell culture.MethodsRat hippocampal neurons in primary culture were exposed to STS (20 μmol/L) and treated with curcumin (20 μmol/L). Cell viability was tested by MTT assay and reactive oxygen species (ROS) were measured using the MitoSOX™ red mitochondrial superoxide indicator. Western blot was used to assess changes in the levels of caspase-3 (Csp3), heat shock protein 70 (Hsp70) and Akt.ResultsThe results showed that curcumin protects against STS-induced cytotoxicity in rat hippocampal neurons. Csp3, Hsp70, Akt and ROS activation may be involved in this protection.ConclusionCurcumin could be a potential drug for combination with STS in cancer treatment to reduce the unwanted cytotoxicity of STS. |
| Keywords: | curcumin staurosporine-induced cytotoxicity hippocampal neurons caspase-3 Akt Hsp70 reactive oxygen species cancer treatment |
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