大黄素对全身炎性反应综合征病人外周血中性粒细胞Fas/FasL、Caspase-3表达的影响

目的 探讨全身炎性反应综合征(SIRS)病人外周血中性粒细胞(PMN)凋亡信号Fas/FasL、Caspase-3表达的动态变化及大黄素对SIRS时PMN凋亡的影响及其作用机制.方法 采集6例健康志愿者及8例SIRS病人(均为急性胰腺炎病人)外周血液并从中分离PMN进行体外培养.分为3个实验组:正常组,SIRS组,大黄素干预组.观察各组PMN的凋亡情况;检测各组PMN Fas/FasL和Caspasc-3表达水平的变化.结果 SIRS组PMN凋亡百分率明显低于正常组(P＜0.05),大黄素干预后SIRS病人PMN凋亡百分率明显增加(P＜0.05);SIRS组PMN Fas和Caspase-3的表达水平明显低于正常组(P＜0.05),大黄素能诱导SIRS病人PMN Fas和Caspase-3的表达(P＜0.05).各组PMN在体外培养24 h后,经Western Blotting没有检测到FasL的表达.结论 SIRS病人外周血PMN凋亡存在异常,且与Fas和Caspase-3的表达降低有关;大黄素能通过诱导Fas和Caspase-3的表达,对SIRS时PMN凋亡延迟具有抑制作用.

Effects of emodin on expression of PMN Fas/FasL,Caspase-3 from peripheral blood in patients with SIRS

Objective To investigate the expression of PMN Fas/FasL,Caspase-3 from peripheral blood in patients with SIRS and observe the effect of emodin on the PMN apoptosis and explore its mechanism.Methods The samples of peripheral blood were collected from 8 patients(all suffer from acute pancreatitis)with SIRS and 6 healthy volunteers.The circulating neutrophils were isolated and cultured.There were three groups of in our study:control group,SIRS group and emodin-treated group.The change of the PMN apoptosis and the expression Ieve]of Fas/FasL.Caspas-3 were observed.Results Compared with healthy volunteers.the percentage of PMN apoptosis significantly reduced in patients with S1RS(P＜0.05).After emodin was used,the percentage of PMN apoptosis in patients with SIRS increased(P＜0.05).Compared with healthy volunteers,the expression of Fas and Caspase-3 reduced in patients with SIRS(P＜0.05).Emodin could significantly induce the expression of Fas and Caspase-3(P＜0.05).After 24 hours of in vitro culture.the expression of FasL was not detected.Conclusion PMN from patients with SIRS shows profoundly delayed rates of apoptosis in vitro compared with PMN from healthy volunteers,and the decreased percentage of PMN apoptosis is associated with the reduced expression of Fas and Caspase-3.Emodin can significantly inhibit the delayed PMN apoptosis in patients with SIRS by inducing the expression of Fas and Caspase-3.