Real-time PCR for detection of Brucella spp. DNA in human serum samples |
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Authors: | C. Debeaumont P. A. Falconnet M. Maurin |
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Affiliation: | (1) Laboratoire de Bactériologie, Centre Hospitalier Universitaire, Faculté de Médecine, Université Joseph Fourier, BP 217, 38043 Grenoble cedex 9, France |
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Abstract: | Presented here are the results of an evaluation of an in-house real-time PCR assay for the rapid and specific diagnosis of human brucellosis. The assay was based on direct amplification from serum samples of a 169-bp portion of bcsp31, a gene found in all Brucella species and biovars. Species specificity and selectivity of this real-time PCR assay were evaluated using genomic DNA from 15 Brucella strains and 42 non-Brucella strains, and the results were 100%. Among 17 culture-proven brucellosis patients, sera from 11 gave a positive amplification signal, corresponding to a sensitivity of 64.7%. In contrast, negative results were obtained for all sera from 60 control patients, corresponding to a specificity of 100%. The results indicate this test is well adapted for definite confirmation of brucellosis cases, when Brucella cultures remain sterile and serological tests demonstrate the presence of cross-reacting antibodies against Brucella sp. and Yersinia enterocolitica O:9 antigens. |
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