Phylogenetic analysis of nonstructural protein 5 (NSP5) gene sequences in porcine rotavirus B strains |
| |
| Authors: | Tohru Suzuki Junichi Soma Ayako Miyazaki Hiroshi Tsunemitsu |
| |
| Affiliation: | 1. Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok 10300, Thailand;2. Division of Molecular Medicine, Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand;3. Chumphae Hospital, Chum Phae, Khon Kaen 40130, Thailand;1. Department of Molecular Epidemiology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan;2. Leading Program, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan;3. Department of Medical Microbiology and Public Health Research Laboratory, Institute of Medicine, Tribhuvan University, Kathmandu, Nepal;4. Department of Health Services, Ministry of Health, Government of Nepal, Kathmandu, Nepal;5. Department of Clinical Infection, Microbiology & Immunology, Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom |
| |
| Abstract: | Porcine rotavirus B (RVB) has frequently been detected in the diarrhea of suckling and weaned pigs. Because it is difficult to propagate RVBs serially in cell culture, little genetic information about RNA segments other than VP7, NSP1 and NSP2 is available for porcine RVBs. We conducted a phylogenetic analysis focusing on nonstructural protein 5 (NSP5) using 22 porcine RVB strains, which were identified in fecal samples collected around Japan. Sequence analysis showed that NSP5 genes of RVBs contain one ORF, in contrast to the corresponding gene of RVAs that have two ORFs. Comparison of NSP5 amino acid sequences from porcine RVBs with other RVBs revealed that eight serine and serine/threonine residues at the N- and C-terminal regions are highly conserved among RVBs. Phylogenetic analysis also indicated the presence of six clusters (H1–H6) including rat, human, porcine and bovine plus ovine clusters with cut-off values of 78% at the nucleotide level. Moreover, the NSP5 genes of porcine RVBs were divided to three clusters. The data presented here demonstrates that several porcine RVBs with distinctive genotypes are circulating among farms throughout Japan. Our findings provide important new insights into the evolutionary dynamics of RVBs. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
