Characterization of the complete mitochondrial genomes of two whipworms Trichuris ovis and Trichuris discolor (Nematoda: Trichuridae) |
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| Authors: | Guo-Hua Liu Yan Wang Min-Jun Xu Dong-Hui Zhou Yong-Gang Ye Jia-Yuan Li Hui-Qun Song Rui-Qing Lin Xing-Quan Zhu |
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| Affiliation: | 1. Institute for Research Promotion, Oita University, Oita 879-5593, Japan;2. Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia;3. Department of Public Health, Faculty of Nursing, Kobe Women''s University, Kobe 650-0046, Japan;4. Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan;5. Department of Orthopaedic Surgery, Ohara General Hospital, Fukushima 960-8611, Japan;6. Department of Diagnostic Pathology, Ohara General Hospital, Fukushima 960-8611, Japan;7. Research Center for the Pacific Islands, Kagoshima University, Kagoshima 890-8580, Japan;8. Agriculture Research Center, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-8666, Japan;9. Department of Parasitology, Kurume University School of Medicine, Kurume 830-0011, Japan;10. Department of Infectious Disease Control, Faculty of Medicine, Oita University, Oita 879-5593, Japan;11. Tropical Infectious Diseases Research and Education Centre (TIDREC), University of Malaya, 50603 Kuala Lumpur, Malaysia;1. Laboratório de Paleoparasitologia e de Biologia Molecular de Parasitos, Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal Fluminense, Rua Professor Hernani Melo 101 São Domingos, Niterói, RJ, 24210-130, Brazil.;2. Laboratório de Paleoparasitologia e de Ecologia Gustavo de Oliveira e Castro, Escola Nacional de Saúde Pública, Fundação Oswaldo Cruz, Rua Leopoldo Bulhões 1480 Manguinhos, Rio de Janeiro, RJ, 21041-210, Brazil. |
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| Abstract: | For many years, whipworms (Trichuris spp.) have been described with a relatively narrow range of both morphological and biometrical features. Moreover, there has been insufficient discrimination between congeners (or closely related species). In the present study, we determined the complete mitochondrial (mt) genomes of two whipworms Trichuris ovis and Trichuris discolor, compared them and then tested the hypothesis that T. ovis and T. discolor are distinct species by phylogenetic analyses using Bayesian inference, maximum likelihood and maximum parsimony) based on the deduced amino acid sequences of the mt protein-coding genes. The complete mt genomes of T. ovis and T. discolor were 13,946 bp and 13,904 bp in size, respectively. Both mt genomes are circular, and consist of 37 genes, including 13 genes coding for proteins, 2 genes for rRNA, and 22 genes for tRNA. The gene content and arrangement are identical to that of human and pig whipworms Trichuris trichiura and Trichuris suis. Taken together, these analyses showed genetic distinctiveness and strongly supported the recent proposal that T. ovis and T. discolor are distinct species using nuclear ribosomal DNA and a portion of the mtDNA sequence dataset. The availability of the complete mtDNA sequences of T. ovis and T. discolor provides novel genetic markers for studying the population genetics, diagnostics and molecular epidemiology of T. ovis and T. discolor. |
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