Giardia duodenalis sub-Assemblage of animal and human origin in horses |
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Authors: | Donato Traversa Domenico Otranto Piermarino Milillo Maria Stefania Latrofa Annunziata Giangaspero Angela Di Cesare Barbara Paoletti |
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Institution: | 1. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan 450002, China;2. Department of Parasitology, Harbin Medical University, Harbin, Heilongjiang 150081, China;3. Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA |
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Abstract: | In order to evaluate infection occurrence and the potential zoonotic role of horse isolates of Giardia duodenalis, 431 individual fecal samples were genetically characterized by PCR tests –coupled sequencing and phylogenetic analysis. Thirty-seven (8.6%) animals resulted infected by different Assemblage. The presence of sub-Assemblage was assessed by characterizing the β-giardin gene for 16 of the 37 positive horses. Ten isolates showed 99.6% to 100% homology with the sub-Assemblage described as B1–2 and B1–6, three Assemblage A showed 99.8% homology with sub-Assemblage A1, while one Assemblage E displayed 98.8% homology with sub-Assemblage E3. Furthermore, one isolate characterized as Assemblage A showed 99.6% homology with the sub-Assemblage B1–2 and one characterized as E was 100% identical with sub-Assemblage B1–6. These results demonstrate the presence of both animal and human sub-Assemblage of G. duodenalis in horses from Italy. Epidemiological and sanitary implications are discussed. |
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