几丁糖预防术后腹腔粘连的实验研究

目的探讨几丁糖对术后腹腔粘连的预防作用.方法将45只大鼠随机分为A、B、C 3组,于腹腔内浆膜损伤部位分别注入1%乳酸液、32%右旋糖酐-70液和2%几丁糖乳酸液各2 ml,术后14 d处死动物,观察各组腹腔粘连状况.结果 3组粘连分级比较有显著性差异(P＜0.01),C组粘连发生率与另2组有显著差异(P＜0.05).光镜下C组炎症反应轻微,纤维增生不明显;电镜下C组纤维细胞分泌胶原能力弱,间皮细胞增生活跃.结论几丁糖可有效降低大鼠术后腹腔粘连的发生和粘连程度,其作用优于右旋糖酐-70,且不影响切口的愈合.

Experimental study on prevention of postoperative peritoneal adhesions by chitosan

Objective: To explore the effect of chitosan on the prevention of postoperative peritoneal adhesion. Methods:Forty-five Wistar rats were randomly divided into group A,B and C; 2 ml of 1% lactic acid, 32% Dextran-70 and 2% Chitosan-lactic acid (CLA) were applied on the present serosal injury respectively during operation. The adhesive degree and biopsy were taken on the 14th postoperative day.Results:There were statistically significant differences among the degrees of adhesions of three groups ( P <0.01), and between the adhesion incidence of group C and that of other two groups ( P <0.05). Mild inflammatory changes and less fibrous proliferation were existed in group C by microscopy and decreased production of collagen (by fibroblast) and active mesothelial cells proliferation were observed in group C under electron microscope. Conclusions:CLA can effectively reduce the incidence and extent of postoperative peritoneal adhesions in rats, which is more satisfactory than Dextran-70. Meantime CLA can't affect the postsurgical wound healing.