| 人参西洋参及其制剂总皂甙紫外分光光度法含量测定方法研究 |
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| 引用本文： | 颜玉贞.人参西洋参及其制剂总皂甙紫外分光光度法含量测定方法研究[J].中药新药与临床药理,1994,5(4):37-40. |
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| 作者姓名： | 颜玉贞 |
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| 作者单位： | 广州市药品检验所 |
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| 摘 要： | 人参西洋参根中人参总皂甙含量测定较为常用的方法有比色法及重量法，本文报道用浓硫酸反应后进行紫外分光光度测定法的方法学研究及其在商品西洋参及其制剂中总皂甙含量测定的应用。对照品人参皂甙Ｒｂ１，Ｒｅ或Ｒｇ１作校正曲线，在８～３０ｕｇ范围均成一通过原点的直线，相关系数０．９９９８～０．９９９９；加样回收率９６．９％。
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| 关 键 词： | ＠人参西洋参／化学，＠总皂甙／分析，紫外分光光度法，＠样品预处理 |
UV-Spectrophotometry for the Determination of Total Saponin content in Radix Ginseng,Radix Ginseng Quinquefolii and Their Preparations |
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| Yan Yuzhen.UV-Spectrophotometry for the Determination of Total Saponin content in Radix Ginseng,Radix Ginseng Quinquefolii and Their Preparations[J].Traditional Chinese Drug Research & Clinical Pharmacology,1994,5(4):37-40. |
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| Authors: | Yan Yuzhen |
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| Abstract: | Golorimetry and gravimetry are commonly used for the determination of totalginsenoside content of Radix Ginseng and Radix Ginseng Quinquefolii. In this study,UV-spectrophotometry was applied for this purpose after the samples were reacted by concentratedsulfuric acid.Control ginsenoside Rb1 or Re or Rg1 was tised to correct the curve.Between the range of8-30 ug,a straight line passing through the original point was shown.The correlation coefficient was0.9998-0.9999 and the rate of recovery was 96.60%. |
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| Keywords: | Radix Ginseng Radix Ginseng Quinquefolii @ total saponins/anaiysisUV-spectrophotometry @ preliminary treatment of sample |
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