| 苦参碱逆转人胃癌细胞株SGC7901/VCR耐药性实验研究 |
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| 引用本文： | 孙亭立,廖传文,曹虹,陈晓亮,饶雪峰.苦参碱逆转人胃癌细胞株SGC7901/VCR耐药性实验研究[J].南昌大学学报(医学版),2010,50(7). |
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| 作者姓名： | 孙亭立 廖传文 曹虹 陈晓亮 饶雪峰 |
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| 作者单位： | 孙亭立(九江市第一人民医院肝胆外科,江西,九江,332000);廖传文,曹虹,陈晓亮,饶雪峰(江西省人民医院普外科,南昌,330006) |
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| 基金项目： | 江西省卫生厅科技计划资助课题 |
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| 摘 要： | 目的 研究苦参碱(Matrine)对人胃癌多药耐药细胞株SGC7901/VCR的多药耐药的逆转效应及其机制.方法 以长春新碱(VCR)诱导的人胃癌多药耐药细胞株SGC7901/VCR为研究对象,MTT法研究苦参碱对体外培养的人胃癌多药耐药细胞株SGC7901/VCR细胞增殖的影响及逆转效应、用间接免疫荧光法经流式细胞仪检测MRP的表达并分析其机制.结果 MTT法显示苦参碱在0.5、1.0、2.0 mg·mL-1浓度下处理SGC7901/VCR细胞72 h后,其生长抑制率依次为10%、15%、32%,且可以逆转SGC7901/VCR对VCR的耐药,呈剂量依赖性,逆转倍数分别为9.4、13.4、24.5倍.0.5 mg·mL-1苦参碱与SGC7901/VCR细胞共同培养24 h后,SGC7901/VCR细胞的MRP表达从(36.00±4.32)%降低至(25.00±3.85)%(P<0.01).结论 苦参碱可以体外逆转人胃癌细胞多药耐药细胞SGC7901/VCR的耐药性,其逆转机制与降低MRP的表达有关.
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| 关 键 词： | 苦参碱 胃癌 多药耐药 |
Reversal Effect of Matrine on Multidrug Resistance in Human Gastric Cancer Cell Line SGC7901/VCR |
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| SUN Ting-li,LIAO Chuan-wen,CAO Hong,CHEN Xiao-liang,RAO Xue-feng.Reversal Effect of Matrine on Multidrug Resistance in Human Gastric Cancer Cell Line SGC7901/VCR[J].Journal of Nanchang University(Medical Science),2010,50(7). |
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| Authors: | SUN Ting-li LIAO Chuan-wen CAO Hong CHEN Xiao-liang RAO Xue-feng |
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| Institution: | SUN Ting-li1,LIAO Chuan-wen2,CAO Hong2,CHEN Xiao-liang2,RAO Xue-feng2(1.Department of Hepatobiliary Surgery,the First People's Hospital of Jiujiang,Jiujiang 332000,China,2.Department of General Surgery,the People's Hospital of Jiangxi,Nanchang 330006,China) |
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| Abstract: | Objective To explore the reversal effect of matrine on multidrug resistance(MDR) in drug-resistant human gastric cancer cell line SGC7901/VCR and its mechanism.Methods The reversal effect of matrine on proliferation of multidrug-resistant SGC7901/VCR cells was determined by using MTT assay,and the expression of MDR-associated protein(MRP) in human gastric cancer cells was detected by flow cytometry.Results The growth inhibition rates of SGC7901/VCR cells were 10%,15% and 32% after 72 hours of treatment with... |
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| Keywords: | matrine gastric cancer multidrug resistance |
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