幽门螺杆菌感染患者血清抗体间接免疫荧光检查方法及其意义

研究幽门螺杆菌(Helicobacter pylori,简称 Hp)感染的一种无创伤性血清免疫荧光抗体检查技术,作为消化性溃疡(Peptic ulcer,PU),慢性胃炎(Chronicgastritis,CG)以及非溃疡性消化不良(Nonulcer dyspepsia,NUD)患者Hp感染的快速诊断和Hp清除与否的指标,并和内镜活检尿素酶法,直接细菌染色法比较,以确定Hp血清免疫荧光抗体的诊断价值。观察对象为内镜诊断的Pu28例,CG122例及正常30例,共180例,均经尿素酶及Hp染色两法测试,结果两法都阳性100例,阴性50例,还有阴性健康者30例。免疫荧光法测试结果,在前两法阳性组的阳性率为95％(95/100),阴性组的阳性率为22％(11/50),阴性健康组的阳性率为3％(1/30)。并对Hp菌血清免疫荧光及非免疫荧光进行对比;前者对Hp诊断特异性强,敏感性高,在粘膜内Hp定位清晰。

Indirect Immunofluorescence Method in Detection of Serum Anti Helicobacter Pylori Antibody and Its Significance

The authors developed an indirect immunofluorescence method in detection of serum antiHelicobacter pyloui (HP)antiboduy, which can be used as a rapid diagnostic method of HP infectionand a criterion of HP clearance in patients with peptic ulcer (PU), chronic gestritis(CG)and anulcerdyspepsia (NUD). The authors conpared this method with one-minute urease test (Imin UT) byendoscopy and tissue smear for HP by Gram stain to determine its diagnostic value. From March, 1990to March, 1991, 180 cases were studied, including PU 28, CG 122, and normal subjects 30, 11 of fhemwere diagnosde by endoscopy and were tested by Imin UT and tissue smear examination. Among all subjects tested by abov two methods, 100 cases were HP positive and another 50 cases and 30 normalsubjects were negativeBy using immuhofluorescence method, HP positive rates were 95% (95/100)in 100 HP positivecases mentioned above, 22%(11/50) in 50HP negative patients and 3%(1/30) in 30 normalsubjects. Comparing the indiredt immunofluorescence method with non -- immunofluorescence methods,the former has a high specificity and sensitivity for determining the HP and also has its practical value.