| 致肾盂肾炎大肠杆菌papA免疫保护作用的初步研究 |
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| 引用本文： | 康宁,王永明,陈锦英,李晓霞. 致肾盂肾炎大肠杆菌papA免疫保护作用的初步研究[J]. 天津医药, 2000, 28(10): 607-609 |
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| 作者姓名： | 康宁 王永明 陈锦英 李晓霞 |
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| 作者单位： | 1. 300070,天津医科大学微生物教研室
2. 300070,天津医科大学毒理教研室 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的：对致肾盂肾炎大肠杆菌ｐ菌毛的结构基因ｐａｐＡ的免疫保护作用进行研究。方法：用ＰＣＲ方法扩增ｐａｐＡ基因，并将５５４ｂｐ扩增产物克隆入真核表达载体ｐｃＤＮＡ３，构建重组质粒ｐＣＴ３７作为ＤＮＡ疫苗，免疫ＢＡＬＢ／ｃ小鼠。结果：全菌ＥＬＩＳＡ测定显示疫苗接种组小鼠血清效价明显高于对照组，分别为１：５７５０和１：４０００（Ｐ〈０．０１）。尿液和肾脏菌落计数在接种组均明显低于对照组（Ｐ〈０．０５），
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| 关 键 词： | 肾盂肾炎 大肠杆菌 papA 免疫保护作用 实验研究 |
Primary Study on Immunoprotective Effects of papA of Uropathogenic Escherichia Coli |
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| KANG Ning,WANG Yongming,CHEN Jinying. Primary Study on Immunoprotective Effects of papA of Uropathogenic Escherichia Coli[J]. Tianjin Medical Journal, 2000, 28(10): 607-609 |
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| Authors: | KANG Ning WANG Yongming CHEN Jinying |
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| Affiliation: | KANG Ning,WANG Yongming,CHEN Jinying;(Department of Microbiology, Tianjin Medical University 300070) |
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| Abstract: | Objective:To investigate the immunoprotective effects of papA of Uropathogenic Escherichia coli(UPEC). Methods: A 554bp long DNA fragment encoding PapA mature polypeptide was amplified using Polymerase Chain Reaction(PCR) ,and was cloned into eukaryotic expressing plasmid pcDNA3. The recombinant plasmid pCT37 was employed as DNA vaccine to immunize BALB/c mice. Results:The antibody titers in experiment group(l:5 750) was significantly higher than that in control group(l:4 000)using whole bacterial ELISA(P<0.01).Both urine and renal colony counts indicated significantly less frequently colonization in experiment group(P<0.05),though there was no significant difference in renal invasion between two groups. Conclusion:papA has a good immunoprotective effect. |
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| Keywords: | Escherichia coli genes structural vaccines DNA immunity |
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