转染BRMS1基因对乳腺癌MDA MB 231细胞体外侵袭力的影响

目的：探讨BRMS1基因对乳腺癌MDA MB 231细胞体外侵袭力的影响。方法：将携带BRMS1基因的真核表达载体pcDNA3.1/myc BRMS1通过脂质体介导稳定转染乳腺癌MDA MB 231细胞；RT PCR检测转染前后细胞中BRMS1mRNA的表达；扫描电镜观察细胞表面超微结构的改变；Boyden小室检测细胞体外侵袭力。结果：转染成功的乳腺癌MDA MB 231细胞其BRMS1mRNA呈高表达；细胞表面超微结构发生改变；体外侵袭力下降。结论:转染BRMS1基因可抑制乳腺癌MDA MB 231细胞的体外侵袭能力。

Effects of BRMS1 gene transfection on invasion potential of breast

Objective:To evaluate the invasion potential of breast cancer cell MDA-MB-231 transfected by BRMS1 gene in vitro.Methods: A eukaryotic expression vector containing BRMS1 gene was transfected into breast cancer cell MDA-MB-231 by lipofectamine,and then RT-PCR was used to detect the expression of BRMS1mRNA in cells before and after transfection,scanning electron microscope to observe the ultrastructure,and Boyden Chamber to assay the change of invasion.Results: After transfection,BRMS1mRNA was up-regulated significantly,the ultrastructure obviously changed,and the ability of invasion significantly dropped off in vitro.Conclusion: BRMS1 gene suppresses the invasion of breast cancer cell MDA-MB-231 in vitro.