温肾咳喘片的主要药效及作用机制研究

目的用动物实验研究温肾咳喘片(蛇床子、厚朴的CO2超临界提取物和甘草水煎醇沉物组成)的止咳、平喘作用,并初步探讨其作用机制。方法用氨水引咳法、酚红排泄实验、组胺-乙酰胆碱引喘、卵白蛋白致喘实验以及肺溢流法等实验研究温肾咳喘片的止咳、平喘作用;用氢化可的松致肾阳虚模型和去势雄性小鼠模型探索其作用机制。结果温肾咳喘片可显著延长氨水致咳小鼠的引咳潜伏期、减少3min内的咳嗽次数,增加小鼠气管段酚红排泌量,明显延长组胺-乙酰胆碱致敏豚鼠的引喘潜伏期、减少抽搐动物数,延长卵白蛋白致喘豚鼠抽搐潜伏期和减少跌倒休克豚鼠数,减少卵白蛋白致敏麻醉大鼠的肺溢流量。温肾咳喘片对氢化可的松致肾阳虚模型小鼠的整体行为活动、体重增长缓慢均有一定的保护作用,并可对去势雄鼠发挥一定的雄激素样生物活性作用。结论温肾咳喘片具有明确的止咳、平喘作用,可能是通过温(肾)阳或雄激素样生物活性实现的。

A Study on the Main Pharmacodynamic Effects of Wenshen Kechuan Tablets and Its Mechanism

Objective To study the effects of Wenshen Kechuan Tablets in relieving cough and asthma, and to make a preliminary investigation of its therapeutic mechanism. Methods The actions of relieving cough and asthma were studied with the cough model induced by ammonia water, asthma model induced by histamine- acetylcholine, asthma model induced by ovalbumin. In addition, the phenolsulfonphthalein excretion and lung overfall were measured. The therapeutic mechanism of Wenshen Kechuan Tablets was studied with the kidney- yang- insufficiency model induced by cortisol and the model of emasculated male mice. Results Wenshen Kechuan Tablets could significantly prolong the cough latent period and cough times within 3 min and increase phenol red secretion from trachea in cough model induced by ammonia water. Wenshen Kechuan Tablets could significantly prolong the asthma latent period and relieve spasm in guinea pigs with histamine- acetylcholine- induced asthma, prolong spasm latent period and reduce shock attack and lung overfall volume in guinea pigs with ovalbumin- induced asthma. Wenshen Kechuan Tablets had a protective effect on whole behavior and body weight increase in model mouse of insufficiency of kidney- yang, and show some androgen- like biological activity in emasculated male mice. Conclusion Wenshen Kechuan Tablets have obvious actions of relieving cough and asthma, the effects may be achieved by warming kidney- yang or by androgen- like biological activity.