应用聚合酶链反应技术检测解脲脲原体

本文就聚合酶链反应（ＰＣＲ）技术检测解脲脲原体（ＵＵ）的方法建立与应用进行了探讨。利用互补于１６ＳｒＲＮＡ基因的一对引物，通过ＰＣＲ对ＵＵ进行检测，扩增片段长度为３９７ｂｐ。与４６例培养对照，培养阳性９例，ＰＣＲ阳性１３例，而且其它相关微生物ＰＣＥ检测无特异性扩增。对临床６２例泌尿生殖道感染患者进行检测，阳性率为１５／６２（２４．２％）。该法具有可靠、简便、快速等优点，用于解脲脲原体感染的临床诊断具有重要价值。

DETECTION OF UREAPLASMA UREALYTICUM BY POLYMERASE CHAING REACTION

polymerase chain reaction(P CR)procedure was develo ped for detection of UreaplasmaUrealyticum(UU).Seqences within a 16S ribosomal RNA gene from UU were used as extensionprimers for the PCR.A single DNA fragment of 397bp was amplified. The culture and the PCRwert carried out for detection of UU In 46 urogenital swabs obtained from patients in our hospital.The culture-positive specunens were 9 cases and they were confirmed by PCR. in 4 of 37 culture-negative specimens,397bp DNA fragment was detected and in the remaining specimens, PCR wasneg ative for UU.No amplified product was detected in Chlamydia trachomatis DNA,N. gonorrhoeae DNA or other bacterial DNAs.UU sequences were de tected by PCR in 15 of62(24.4%) clinical specimens.The method was roliable and simple, and it could be applied fordiagnosis of UU infection.