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Cloning hybridoma cells by limiting dilution |
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| Authors: | Joan C. Rener Bruce L. Brown Roland M. Nardone |
| Affiliation: | (1) Hazleton Biotechnologies Corporation, 9200 Leesburg Turnpike, 22180 Vienna, Virginia, USA;(2) Center for Advanced Training in Cellular and Molecular Biology, The Catholic University of America, Washington, DC, USA |
| Abstract: | Summary A method is described for cloning hybridoma cells by limiting dilution. This technique utilizes a feeder layer of murine splenocytes to enhance the growth of clones. |
| Keywords: | hybridoma cells cloning feeder layer |
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