丙型肝炎患者血清IL-17与ALT等指标相关性分析

目的了解慢性丙型肝炎患者和对照组血清ALT、AST、TBil、IL-17水平以及在丙型肝炎的不同发展阶段的变化特点,探讨病毒载量与IL-17、ALT、AST、TBil的相关性。方法 85例丙型肝炎患者其中包括24例丙型肝炎肝硬化患者和61例慢性丙型肝炎患者,30名健康人作为对照。所有丙型肝炎患者抗-HCV抗体检测S/CO〉3.8（排除抗-HCV假阳性患者）,研究对象分为4组。采用全自动生化分析仪检测所有研究对象的ALT、AST、TBil的水平,采用酶联免疫吸附实验-双抗体夹心法（ELISA）检测所有研究对象血清中抗-HCV抗体和细胞因子IL-17水平。采用荧光定量聚合酶链反应（RT-PCR）检测HCV-RNA的病毒载量。结果（1）通过各组间多重比较,丙型肝炎患者各组IL-17水平显著高于健康对照组（P〈0.05）,差异有统计学意义,在病毒载量〉1×10^3组IL-17水平与ALT、AST水平变化有很好的相关性。（2）按照病毒载量HCV-RNA高低分组,病毒载量与IL-17水平呈正相关,随着病毒载量的升高IL-17水平也随之升高。结论患者组IL-17、ALT、AST、TBil的检测水平升高可能与患者肝细胞的炎性损伤有关,并且IL-17在一定程度上能够反映肝脏的损伤程度。在病毒检测阳性的患者中病毒载量与IL-17水平呈正相关。

Correlation between serum IL-17 and ALT,AST,T-Bil in patients with HCV infection

Objective The understand the characteristics of changes of ALT,AST,T-Bil and IL-17 in different stages of hepatitis C disease development. Methods Eighty-five patients with hepatitis C infection（24cases of hepatitis C cirrhosis and 61 cases of chronic hepatitis C infection）were investigated and 30 healthy persons were as controls.The automatic biochemical analyzer was used to test the ALT,AST and T-Bil levels and the ELISA method was used to test the serum anti-HCV antibody and cytokine IL-17 levels.The RT-PCR was used to test the HCV-RNA virus load. Results The IL-17 levels of all hepatitis C patients were significantly higher than that of the healthy control group（P〈0.05）.In the group of patients with more than 1×103 virus load,the IL-17 level had a good correlation with ALT,AST and T-Bil levels.The virus load was positive correlation with the IL-17 level and the IL-17 level increased with the increasing of the virus load. Conclusions The increasing of IL-17,ALT,AST and T-Bil levels may be related to the hepatic cell inflammatory injury of patients and can reflect the liver injury degree in some extent.