TAP基因转染提高其在肿瘤细胞系的表达

目的　筛选TAP1、TAP2表达下调的肿瘤细胞系 ,将TAP1、TAP2基因分别转染其表达下调的肿瘤细胞系 ,检测基因转染后的mRNA表达水平。方法　用RT -PCR方法检测肺腺癌细胞系Anip 973,AGZY83a ,LH - 7,BE - 1,胃癌细胞系BGC - 82 3TAP1及TAP2mRNA水平 ,筛选TAP1、TAP2表达下调的肿瘤细胞系。用脂质体法 (LipofectaminTM2 0 0 0 )将TAP1、TAP2分别转染人肺腺癌细胞系Anip 973,经G4 18筛选 4～ 6周 ,通过RT -PCR检测TAP1及TAP2的表达。结果　Anip 973,AGZY 83a细胞TAP1、TAP2mRNA表达下调 ,LH - 7,BE - 1TAP1PCR产物均为 2条带 ,BE - 1TAP 2mRNA表达下调 ,而LH - 7与B细胞相同。转染后的Anip 973细胞TAP1、TAP2的mRNA表达明显增加。结论　基因转染可恢复肿瘤细胞TAP1及TAP2的表达 ,为增强MHCⅠ类分子提呈肿瘤抗原奠定了基础

Upregulation of TAP Expression in Tumor Cell Line By Transfection with TAP Gene

Objective To screen the tumor cell line with downregulation of TAP 1 from lung adenocarcinoma cell lines Anip973, AGZ83a, LH-7,BE-1 and gastric carcinoma cell line BGC-823 used by TAP gene transfection. The TAP mRNA level was investigated. Methods The TAP 1 and TAP 2 recombinant expression vector by binding lipofectamin 2000 was transfected in Anip973 cells. The expression of TAP 1 and TAP 2 were detected by RT-PCR assay. Results TAP 1 and TAP 2 mRNA level were decreased in Anip973 and AGZY83a cells. The expression of TAP 2 in BE-1 cells was decreased. RT-PCR results showed the TAP 1 or TAP 2 mRNA expression of Anip973 cells was increased. Conclusion These study suggested that the TAP expression could be enhanced by TAP gene transfer and layed the foundation for increasing tumor antigen presentation by class MHC molecule pathway.