T-STAR基因对人结肠癌细胞系HCT-116生长增殖的影响

目的　探讨正、反义T- STAR(testes signaltransductionandactivatorofRNA,T -STAR)基因对结肠癌细胞HCT- 116 生长增殖的影响。方法　用脂质体介导法分别将正、反义T -STAR基因转入HCT -116细胞中,用Westernblot检测T -STAR 蛋白表达水平,用细胞动力学检测HCT- 116细胞的生长、增殖变化。结果　转染正义T -STAR基因后,HCT -116细胞的T STAR蛋白表达水平增加,但细胞生长速度变慢、增殖能力下降;转染反义T -STAR基因后,HCT- 116细胞的T -STAR蛋白表 达水平下降,但细胞生长速度变快、增殖能力增强。结论　T- STAR基因可抑制结肠癌细胞HCT- 116的生长、增殖。

Effects of sense and antisense T-STAR gene on colon cancer cell line HCT-116

Objective To investigate the effects of the sense and antisense testes-signal transduction and activator of RNA (T-STAR) gene on the colon cancer cell line HCT-116. Methods The sense and antisense T-STAR gene was stably transfected into HCT-116 cells with lipofectamine. The expression level of T-STAR in those cells was detected by Western blotting and the growth velocity and proliferation of those cells by cytokinetics. Results The growth velocity and proliferation decreased after transfection of the sense T-STAR gene, but increased after transfection of the antisense T-STAR gene. Conclusion T-STAR gene can inhibit the growth velocity and proliferation of HCT-116 cells.