多发性硬化患者T细胞亚群与中医证候的相关性研究

目的研究多发性硬化患者T细胞亚群的变化和中医证候以及病期的关系，探讨多发性硬化患者T细胞亚群的变化在中医证候形成过程中的意义。方法将59例多发性硬化患者进行中医辨证分型和疾病分期，且全部进行规范的中西医治疗，并统计各类患者的T细胞亚群情况。结果多发性硬化患者在激素治疗期的CD4^＋T细胞亚群均值与其他病期比较显著增高（P〈0．05）；在肝肾阴虚型激素治疗期中，CD4^＋T细胞亚群的均值与其他各病期比较显著增高（P〈0．05），CD3^＋CD19^-T细胞亚群的均值与稳定期比较显著增高（P〈0．05）；激素治疗后CD3^＋CD19^-T细胞均值与激素治疗期相比显著降低（P〈0．05）。结论在肝肾阴虚型与其他证型比较，CD4^＋T细胞的水平下降更快，说明肝肾阴虚型患者的炎症消退更明显。可将诱导性CD4^＋T细胞和调节性CD4^＋CD25^high曲T细胞做为判断治疗疗效的指标，并参考其变化来调整治疗方案。

Correlative Research between T - Cell Subsets and Chinese Medicine Patterns/Syndromes in the Patients of Multiple Sclerosis

Objective To study the correlation of T - cell subsets with Chinese medicine patterns/ syndromes and the disease stages for the patients of multiple sclerosis （MS）and explore the significance of the changes in T - cell subsets in the formation of Chinese medicine patterns/syndromes in MS patients. Methods Fifty - nine cases of MS were differentiated in terms of Chinese medicine patterns/syndromes and disease stages. All of the cases were treated with standard integrated Chinese and western medicine. T - cell subsets were analyzed statistically for all kinds of MS patients. Results The mean value of CD4^＋ T - cell subsets was increased significantly for MS patients at the hormone treatment stage as compared with the other stages （ P 〈 0.05 ）. For the patients of liver and kidney yin deficiency at hormone treatment stage, the mean value of CD4^ ＋ T - cell subsets was increased significantly as compared with the other stages （ P 〈 0.05 ） and the mean value of CD3^＋ CD19^ - T - cell subset was increased significantly as compared with that in the stable stage （ P 〈 0.05 ）. After hormone treatment, the mean value of CD3 ^＋ CD19^ - T cells was reduced significantly as compared with that in the hormone treatment stage （ P 〈 0.05 ）. Conclusion Compared with the other patterns/syndromes of Chinese medicine, the level of CD4^ ＋ T cells is reduced much quickly in the patients of liver and kidney yin deficiency. It indicates that the inflammatory symptoms of the patients of liver and kidney yin deficiency are relieved much more apparently. The inductive CD4 ^＋ T cells and regulatory CD4 ＊ CD25hish T cells can be the indexes for the effect judgment and the adjustment of therapeutic program.