| 移植胚鼠神经干细胞对大鼠脊髓损伤后红核神经元损伤的影响 |
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| 引用本文： | 孔令胜,聂冬丽,张军臣,张浩,许华.移植胚鼠神经干细胞对大鼠脊髓损伤后红核神经元损伤的影响[J].基础医学与临床,2010,30(4):394-397. |
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| 作者姓名： | 孔令胜 聂冬丽 张军臣 张浩 许华 |
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| 作者单位： | 济宁医学院,附属医院,神经外科,山东,济宁,272029 |
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| 摘 要： | 目的研究神经干细胞(NSCs)移植对大鼠脊髓损伤(SCI)后红核神经元的作用。方法5-溴脱氧尿嘧啶核苷(BrdU)法标记处于对数生长期的NSCs,采用电控脊髓损伤打击装置制作大鼠脊髓损伤模型。实验分为3组:NSCs组、SCI组和假手术组(Sham组)。SCI后3 d进行NSCs移植,用免疫组化法观察移植细胞的存活及迁移情况,用辣根过氧化物酶(HRP)逆行示踪技术标记红核神经元,并用四甲基联苯胺(TMB)呈色反应显示红核脊髓束神经元的存活情况,用行为学(BBB)评分法观察大鼠瘫痪肢体的恢复情况。结果在损伤脊髓区域可检测到BrdU标记的阳性NSCs,中脑HRP标记红核神经元数目明显多于SCI组(P<0.01),BBB评分亦明显高于SCI组(P<0.01)。结论体外培养的胚胎大鼠NSCs在移植到脊髓损伤区域后可存活和迁移,对SCI后中脑红核神经元具有保护作用,从而促进了大鼠肢体功能的恢复。
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| 关 键 词： | 脊髓损伤 神经干细胞 红核神经元 大鼠 |
| 收稿时间： | 2009-5-19 |
| 修稿时间： | 2009-10-28 |
Effects of embryonic neural stem cells on trauma of red nucleus neurons of the rats with spinal cord injury after transplantion |
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| KONG Ling-sheng,NE Dong-li,ZHANG Jun-chen,ZHANG Hao,XU Hua.Effects of embryonic neural stem cells on trauma of red nucleus neurons of the rats with spinal cord injury after transplantion[J].Basic Medical Sciences and Clinics,2010,30(4):394-397. |
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| Authors: | KONG Ling-sheng NE Dong-li ZHANG Jun-chen ZHANG Hao XU Hua |
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| Institution: | Dept.of Neurosurgery;Affiliated Hospital of Jining Medical College;Jining 272029;China |
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| Abstract: | Objective To study the effects of embryonic neural stem cells transplantion on trauma of red nucleus neurons of the rats with spinal cord injury.Methods NSCs in logarithmic phage were labeled with BrdU,a Sprague Dawley rat mode of spinal cord injury(SCI) was developed with electrocircuit control spinal cord injuring device.Thirty SD rats were randomly divided into three groups: sham group,SCI group and NSC group.The NSCs were transplanted into injured site three days after SCI.Then NSCs labeled with Brdu we... |
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| Keywords: | spinal cord injury(SCI) neural stem cells (NSCs) red nucleus (RAN) rat |
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