| 小分子化合物E-039抑制胃癌细胞迁移侵袭及其分子机制 |
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| 引用本文： | 张健,刘彤,李瑞娟,刘奇,李丹妮,李丰. 小分子化合物E-039抑制胃癌细胞迁移侵袭及其分子机制[J]. 解剖科学进展, 2014, 0(2): 105-108,111 |
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| 作者姓名： | 张健 刘彤 李瑞娟 刘奇 李丹妮 李丰 |
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| 作者单位： | [1]中国医科大学基础医学院细胞生物教研室教育部医学细胞生物学重点实验室,沈阳110001 [2]沈阳药科大学制药工程学院,沈阳110016 [3]中国医科大学附属第一医院肿瘤内科,沈阳110001 |
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| 基金项目： | 国家自然科学基金资助项目（No.81230077.31371424,81302127） |
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| 摘 要： | 目的研究小分子化合物E-039对人胃癌细胞增殖、迁移侵袭的影响及其分子机制。方法用MTT检测不同浓度小分子化合物E-039对胃癌细胞BGC823和MKN-45增殖能力的影响。分别用0、20、40、60μmol/L浓度的E-039处理BGC823和MKN-45两种胃癌细胞,应用Transwell小室检测其对上述细胞迁移侵袭能力的抑制作用,Western Blot检测化合物对金属基质蛋白酶2(MMP2)表达及丝切蛋白(Cofilin)磷酸化水平的影响。结果化合物E-039对于BGC823和MKN-45两种胃癌细胞的增殖能力无明显影响,却能够以剂量依赖的方式抑制BGC823及MKN-45细胞的迁移和侵袭。Western Blot结果显示E-039下调胃癌细胞BGC823中MMP2的表达及Cofilin的磷酸化水平。结论化合物E-039能够通过下调胃癌细胞中MMP2的表达及Cofilin的磷酸化水平,抑制胃癌细胞BGC823、MKN-45的迁移和侵袭。
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| 关 键 词： | E-039 人胃癌 侵袭 金属基质蛋白酶2 Cofilin |
The effect and mechanism of E-039 inhibiting the migration and invasion of gastric cancer cells |
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| ZHANG Jian,LIU Tong,LI Rui-juan,LIU Qi,LI Dan-ni,LI Feng. The effect and mechanism of E-039 inhibiting the migration and invasion of gastric cancer cells[J]. Progress of Anatomical Sciences, 2014, 0(2): 105-108,111 |
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| Authors: | ZHANG Jian LIU Tong LI Rui-juan LIU Qi LI Dan-ni LI Feng |
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| Affiliation: | 1. Department of Cell Biology, Key Laboratory of Medical Cell Biology of Public Health Ministry of China, China Medical University, Shenyang 110001; 2. School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016 3. Medical Oncology Department of First Affiliated Hospital, China Medical University, Shenyang 110001, China ) |
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| Abstract: | Objective To observe the effect and mechanism of E-039 on the proliferation, migration and invasion of human gastric cancer cells. Methods WM＇fT method was used to study the effect of E-039 on the proliferation of BGC823 and MKN-45 cells. The Transwell was used to check the migration and invasion of BGC823 and MKN-45 cells with 0, 20, 40, 60 μmol/L E-039. Western Blotting assay was adopted to identify the effect of E-039 on the expression of matrix metalloproteinase 2（MMP2） and phosphorylated Cofilin. Results E-039 did not affect the proliferation of BGC823 and MKN-45 cells, but could dramatically inhibit the migration and invasion ability of BGC823 and MKN-45 cells in a dose- dependent manner. Furthermore, E-039 could significantly down-regulate the expression levels of MMP2 and phosphorylated Cofilin in BGC823 cells. Conclusion The inhibition of E-039 on the migration and invasion capacity of BGC823 and MKN-45 cells might be related to the down-regulation of the expression levels of MMP2 and phosphorylated Cofilin. |
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| Keywords: | E-039 human gastric cancer invasion matrix metalloproteinase 2 Cofilin |
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