山东地区不同人群中SEN病毒感染状况

目的　了解山东地区不同人群中SEN病毒 (SENV)感染的状况与其致病性。方法应用逆转录 聚合酶链反应法 (RT PCR)检测了 2 5 4份不同人群和肝病患者血清中SENVDNA ,并对 17例不明原因ALT增高 ,单独SENVDNA阳性的非甲 戊型肝炎患者行肝活检。结果SEN VDNA总阳性检出率为 2 0 .9%( 5 3 / 2 5 4) ,其中非甲 戊型肝炎血清检出率最高为 5 9.6%( 2 8/ 47) ,明显高于健康献血员 3 .2 %( 1/ 3 1)、非肝病患者 5 .5 %( 3 / 5 5 )、输血后丙型肝炎 42 .9%( 12 / 2 8)、血液透析患者 15 .8%( 2 / 13 )和慢性乙肝患者检出率 8.8%( 7/ 80 ) ,SENV在各种人群中存在着显著性差异 (P <0 .0 1)。SENV感染其主要病理学改变为汇管区炎症 ,淋巴细胞集聚 ,碎小坏死 ,小叶内病变较轻 ,呈散在和点状坏死 ,而桥样坏死 ,脂肪性变较少。结论　本研究表明山东地区不同人群及肝病患者中存在着较高SENV感染率 ;SENV感染可能具有嗜肝性 ;而且可能与肝功损害有关 ,是引起非甲 戊型肝炎重要病原 ,输血后丙肝和有血液透析史患者SENV感染率较高 ,提示对献血员还应严格筛选

Infective Condition of SEN Virus(SENV) in Different Groups of People in Shandong

Objective To know the infective status and pathogenesis of SEN virus in different groups of people in Shandong area. Methods SENVDNA were amplified and detected by RT PCR from 254 sera of patients in different groups of people in Shandong area.17 non A E hepatitis patients with ALT increasing but SENVDNA positive received the liver biopsies.Results SENVDNA positive rate was totally 20.9%(53/254), among these groups,the group of non A E hepatitis had the highest SENVDNA positive rate 59.6%(28/47).However, in the groups of health blood donor the positive rate is 3.2%(1/31) and that of non liver diseases is 5.5%(3/55).The difference was significan.The SENVDNA positive rate in the group of post sfusion hepatitis C 42.9%(12/28) was higher than that 15.8%(2/13) in hemodialysis patients and that 8.8%(7/80) in chronic hepatitis B.There were significan higher differences among different groups ( P <0.01).The main lesion was periportial infiammation, dense lymphoid aggregates and piecemeal necrosis. Intralobular injury of hepatocytes is rather mild which takes on scattered spottynecrosis;Bridging necrosis and fatty degenerateion were not common but respectively.Conclusion The SENV infection rate is high indifferent groups of people in Shandong.SENV infection might be hepatotropic,probably it was correlated with the lesion of the liver and it's the important pathogen of non A E hepatitis.The post transfusion hepatitis C and hemodialysis patients had the higher SENV infection rate,which suggested that blood donors biopsies should be strictly screened.