| 局灶性脑缺血时血红素氧合酶-1 mRNA的表达 |
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| 引用本文： | 符荣,赵甲山,赵洪洋,朱贤立,陈衔城,金复生.局灶性脑缺血时血红素氧合酶-1 mRNA的表达[J].中国神经科学杂志,2004,20(2):145-149. |
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| 作者姓名： | 符荣 赵甲山 赵洪洋 朱贤立 陈衔城 金复生 |
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| 作者单位： | [1]华中科技大学同济医学院附属协和医院神经外科,湖北武汉430022 [2]复旦大学附属华山医院神经外科,湖北武汉430022 [3]复旦大学附属金山医院中心实验室,湖北武汉430022 |
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| 摘 要： | 目的 观测永久性脑缺血后一氧化碳限速酶一血红素氧合酶-1(HO-1)mRNA表达的变化规律。方法 在建立MCAO局灶性脑缺血模型基础上，采用半定量RT-PCR技术观察并测定脑缺血后不同时相HO-1 mRNA的相对表达量。结果脑缺血后1h即有HO-1 mRNA的表达，随时间延长而逐渐升高，12h达最高，以后逐渐下降，至7d时仍有表达。结论 脑缺血后HO-1 mRNA表达变化是缺血脑组织损伤后重要的自身恢复机制之一。
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| 关 键 词： | 局灶性脑缺血 血红素氧合酶-1mRNA 表达 一氧化碳 基因表达 |
Time-phase changes of heme oxygenase-1 gene expression after focal cerebral ischemia in rats |
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| FU Rong ,ZHAO Jia-shan ,ZHAO Hong-yang ,ZHU Xian-li ,CHEN Xian-cheng ,JIN Fu-sheng.Time-phase changes of heme oxygenase-1 gene expression after focal cerebral ischemia in rats[J].Neuroscience Bulletin,2004,20(2):145-149. |
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| Authors: | FU Rong ZHAO Jia-shan ZHAO Hong-yang ZHU Xian-li CHEN Xian-cheng JIN Fu-sheng |
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| Institution: | FU Rong 1,ZHAO Jia-shan 1,ZHAO Hong-yang 1,ZHU Xian-li 1,CHEN Xian-cheng 2,JIN Fu-sheng 3 |
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| Abstract: | Objective To observe the change and the law of heme oxygenasr-1 (HO-1) mRNA expression after permanent cerebral ischemia in rats. Methods The model of middle cerebral artery occlusion in rats was established and the expression of HO-1 mRNA was detected by semi-quantity RT-PCR method. Results The expression of HO-1mRNA in occluded sides increased 1 h after the occlusion, peaked at 12 h, then decreased gradually, lasted till the 7 th day. Conclusion Quick, long-lasting expression of HO-1 mRNA is evidently with significant physiological consequences in the recovery of neuronal tissue from ischemic injury in focal cerebral ischemia in rats. |
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| Keywords: | focal cerebral ischemia carbon monoxide heme oxygenase-1 gene expression |
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