| HPLC测定铁皮石斛饮料中人参皂苷Rb1的含量 |
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| 引用本文： | 王增,祝宇华,杨兵勋,陈立钻,楼正家,沈春香,杨芳亮,夏克中,贾婷婷.HPLC测定铁皮石斛饮料中人参皂苷Rb1的含量[J].中国现代应用药学,2010,27(2):1206-1208. |
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| 作者姓名： | 王增 祝宇华 杨兵勋 陈立钻 楼正家 沈春香 杨芳亮 夏克中 贾婷婷 |
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| 作者单位： | 广东药学院分析化学教研室; |
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| 基金项目： | 广东省自然科学基金资助项目(8451022401000544); 广东药学院人才引进项目资助 |
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| 摘 要： | 目的根据羟自由基与水杨酸(SA)氧化反应的动力学过程,建立动力学分光光度法测定中药多糖对羟自由基(.OH)的清除率。方法应用紫外分光光度法,建立.OH与SA反应的动力学曲线,根据动力学曲线斜率的变化测定中药陈皮和灵芝多糖对.OH的清除率,并用维生素C进行方法的重复性与可靠性验证。结果测定陈皮多糖对.OH的50%清除率(IC50)为232.6 mg.L-1;灵芝IC50为0.856 1 g.L-1;维生素C的IC50为0.254 4 mmol.L-1,RSD为0.2%(n=5)。结论此方法灵敏可靠,可用于中药多糖成分对.OH清除率的测定。
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| 关 键 词： | 动力学分光光度法 羟自由基 水杨酸 清除率 |
Determination of the Content of Ginsenoside Rb1 in Tiepishihu Beverage by HPLC |
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| WANG Zeng,ZHU Yuhu,YANG Bingxun,CHEN Lizuan,LOU Zhengji,SHEN Chunxiang,YANG Fangliang,XIA Kezhong,JIA Tingting.Determination of the Content of Ginsenoside Rb1 in Tiepishihu Beverage by HPLC[J].The Chinese Journal of Modern Applied Pharmacy,2010,27(2):1206-1208. |
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| Authors: | WANG Zeng ZHU Yuhu YANG Bingxun CHEN Lizuan LOU Zhengji SHEN Chunxiang YANG Fangliang XIA Kezhong JIA Tingting |
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| Institution: | FENG Huiping,DU Ruining,ZHANG Zhenying(Department of Analytical Chemistry,Guangdong Pharmaceutical University,Guangzhou,China |
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| Abstract: | OBJECTIVE The reaction dynamic of hydroxyl free radical(.OH) and salicylic acid was explored,the elimination ratios of polysaccharide extractive of Chinese traditional medicine to.OH was determined by kinetic spectrophotometry.METHODS The dynamics curve of the reaction.OH and salicylic acid was built by UV method.The elimination ratio of tangerine pee polysaccharide and ganoderma lucidum polysaccharide to.OH was detemined based on the slope change of the graph.Repetitiveness and reliability of this method w... |
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| Keywords: | kinetic spectrophotometry hydroxyl free radical salicylic acid elimination ratio |
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