| 尿液HCMV-DNA和血清HCMV-IgM抗体诊断人巨细胞病毒感染的价值 |
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| 引用本文： | 齐莹,何蓉,马艳萍,孙峥嵘,刘庆,王继东,吉耀华,阮强.尿液HCMV-DNA和血清HCMV-IgM抗体诊断人巨细胞病毒感染的价值[J].山东医药,2009,49(19):18-19. |
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| 作者姓名： | 齐莹 何蓉 马艳萍 孙峥嵘 刘庆 王继东 吉耀华 阮强 |
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| 作者单位： | 中国医科大学附属盛京医院,沈阳,110004 |
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| 摘 要： | 目的提高人巨细胞病毒（HCMV）感染的诊断水平。方法对960例临床疑诊HCMV感染患儿分别应用FQ—PCR、CLIA法检测尿液HCMV—DNA及血清HCMV-IgM抗体水平。结果尿液HCMV-DNA阳性478例，HCMV-IgM抗体阳性206例；二者均阳性191例。478例尿液HCMV—DNA阳性者中，血清IgM抗体阳性HCMV-DNA拷贝数对数均值高于阴性者（P〈0．01）。结论尿液HCMV-DNA定量和血清HCMV-IgM抗体联合检测有助于HCMV感染的诊断。
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| 关 键 词： | 人巨细胞病毒 婴儿 荧光定量聚合酶链反应 化学发光免疫分析法 |
Dingnostic significance of detecting urine HCMN-DNA and serum HCMV-IgM |
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| QI Ying,HE Rong,MA Yan-ping,SUN Zheng-rong,LIU Qing,WANG Ji-dong,JI Yao-hua,RUAN Qiang.Dingnostic significance of detecting urine HCMN-DNA and serum HCMV-IgM[J].Shandong Medical Journal,2009,49(19):18-19. |
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| Authors: | QI Ying HE Rong MA Yan-ping SUN Zheng-rong LIU Qing WANG Ji-dong JI Yao-hua RUAN Qiang |
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| Institution: | ( Shengjing Hospital of China Medical University, Shenyang 110004, P. R. China) |
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| Abstract: | Objective To improve the diagnostic level of HCMV infection. Methods 960 suspected cases with HCMV infection were selected , and the urine HCMV-DNA and serum HCMV-IgM of which were detected by Fluorescent Quantitative PCR（FQ-PCR） and LIAISON Chemilumineseent Immanoassay （CLIA） respectively. Results 478 eases were positive for HCMV-DNA ,and 206 eases were positive for HCMV-IgM. 191 cases were positive for two indexes. The viral DNA load（logarithmic mean）in serum HCMV-IgM positive cases was higher than those in negative eases （P 〈 0. 01 ）. Conclusion Combined detection of urine HCMV-DNA and serum HCMV-IgM is benefical to the diagnosis of HCMV infection. |
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| Keywords: | human eytomegalovirus infants fluorescent quantitative PCR ehenfilumineseent immunoassay |
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