高效液相色谱法测定蒲公英注射液中咖啡酸含量

目的建立测定蒲公英注射液中咖啡酸含量的高效液相色谱法。方法使用XTerra MSC18（4．6mm×150mm，5μm）色谱柱，以甲醇．乙腈一磷酸盐缓冲液（18：2：80，V／V）为流动相，流速1．0ml·min-1，柱温25℃；在323nm检测波长条件下，考察方法的专属性、准确度、精密度、稳定性等。结果咖啡酸进样量在0．02～0．50μg范围内与峰面积呈现良好的线性关系，回归方程为Y=1695．52X＋6．1582×106（r2=0．9999）；平均加样回收率为99．04％，RSD=0．75％。结论该法简单、准确、稳定，可以用于蒲公英注射液中咖啡酸的含量测定。

Determination of caffeic acid in taraxacum injection by HPLC

Objective To establish an HPLC method for the determination of caffeine acid in taraxacum injection.Methods The HPLC method was validated using a XTerra MS C18 analytical column(4.6 mm×150 mm,5 μm) and the mobile phase of methanol-acetonitrile-phosphate buffer(18∶ 2∶ 80,V/V) at a flow rate of 1.0 ml·min-1 with UV detection at 323 nm.The column temperature was kept at 25℃.The validation parameters including precision,repeatability,stability etc were observed.Results The linear range of caffeic acid was from 0.02 to 0.50 μg.regression equation was Y=1 695.52X+6.158 2×106(r2= 0.999 9).The average recovery was 99.04%,and RSD was 0.75%.Conclusion This method is simple,convenient,accurate,and was good stability with good repeatability,which can be used for the determineation of caffeine acid in taraxacum injection.