|
|||||
|
|
| 慢性上颌窦炎患者耐甲氧西林金黄色葡萄球菌的检测与耐药性研究 | |
| 引用本文： | 刘锋,周水森,张速勤,邓群,张乐之,许育,王靖.慢性上颌窦炎患者耐甲氧西林金黄色葡萄球菌的检测与耐药性研究[J].临床耳鼻咽喉头颈外科杂志,2001,15(8):341-343. |
| 作者姓名： | 刘锋 周水森 张速勤 邓群 张乐之 许育 王靖 |
| 作者单位： | 1. 第二军医大学附属长海医院耳鼻咽喉科 2. 湖北宜昌市第一人民医院检验科第二军医大学 3. 第二军医大学附属长海医院检验科 |
| 摘 要： | 目的 :研究慢性上颌窦炎患者耐甲氧西林金黄色葡萄球菌 (MRSA)的分布和对常见抗菌药物的耐药情况。方法 :采用多重聚合酶链反应 (PCR)同时检测金葡菌的耐药基因 mec A及辅助基因 fem A ;药敏试验采用纸片扩散法。结果 :fem A基因为金葡菌的特有基因 ,mec A基因在 MRSA中检出率为 96 .2 % (5 1/ 5 3)。 MRSA除对万古霉素敏感外 ,对其它抗菌药物都有耐药。结论 :多重 PCR能快速而准确地鉴定 MRSA;MRSA对多种抗菌药物有耐药性。 |
| 关 键 词： | 耐甲氧西林金黄色葡葡球菌 上颌窦炎 聚合酶链反应 mecA基因 femA基因 |
| 文章编号： | 1001-1781(2001)08-0341-03 |
| 修稿时间： | 2001年1月5日 |
Research of drug resistance and detection of methicillin-resistant staphylococcus aureus in chronic maxillary sinusitis |
|
| LIU Feng,ZHOU Shuimiao,ZHANG Suqin,DENG Qun,ZHANG Lezhi,XU Yu,WANG Jin.Research of drug resistance and detection of methicillin-resistant staphylococcus aureus in chronic maxillary sinusitis[J].Journal of Clinical Otorhinolaryngology,2001,15(8):341-343. | |
| Authors: | LIU Feng ZHOU Shuimiao ZHANG Suqin DENG Qun ZHANG Lezhi XU Yu WANG Jin |
| Institution: | Department of Otolaryngology, Changhai Hospital, Second Military Medical University, Shanghai 200433. |
| Abstract: | Objective:To investigate the detection method of methicillin resistant staphylococcus aureus (MRSA) in chronic maxillary sinusitis and the situation of drug resistance to some common antibiotics.Method:Using multiplex polymerase chain reaction technique to detect the drug resistant gene (mecA)of MRSA and specific gene(femA) of staphylococcus aureus at the same time. Drug resistant tests were performed with disk diffusion (K B) method. Result:femA is a specific gene of staphylococcus aureus, the detective ratio of mecA in MRSA is 96.2 % (51/53). Vancomycin is the only antibiotic which is sensitive to MRSA, while others are not. Conclusion:MRSA can be rapidly and specifically identified with multiplex PCR.There are drug resistance to many antibiotic drugs for MRSA. |
| Keywords: | Methicillin resistant staphylococcus aureus Maxillary sinusitis Polymerase chain reaction mecA gene femA gene |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |