| 人巨细胞病毒AD169株引起小鼠脑部畸形的初步探讨 |
| |
| 引用本文： | 谢妮,陈利玉,戴橄,邬国军,罗敏华,袁建辉.人巨细胞病毒AD169株引起小鼠脑部畸形的初步探讨[J].湖南医科大学学报,2003,28(3):243-246. |
| |
| 作者姓名： | 谢妮 陈利玉 戴橄 邬国军 罗敏华 袁建辉 |
| |
| 作者单位： | [1]中南大学湘雅医学院微生物学教研室，长沙410078 [2]中南大学湘雅医学院肿瘤研究所，长沙410078 |
| |
| 摘 要： | 目的：探讨人巨细胞病毒(human cytomegalovirus，HCMV)感染昆明小鼠的敏感性，建立HCMV致畸的动物模型。方法：昆明系小鼠40只，随机分为两组：对照组(8只)和接种病毒组(32只)，病毒感染途径为脑内注射。在接种病毒7，15，30，60d后不同时期分别以病理学方法观察动物脑部发育特点，以免疫组织化学方法检查病毒抗原，PCR方法检测动物脑组织中HCMV基因。结果：接种HCMV AD169株的小鼠脑组织在不同时期发生了病理改变和脑部发育畸形，用免疫组织化学方法和PCR方法在脑组织细胞内检获HCMV抗原和基因。结论：HCMV ADl69株可引起小鼠脑部发育畸形，这对研究HCMV感染致畸的机制提供了有价值的参考依据。
|
| 关 键 词： | 人巨细胞病毒 AD169株 小鼠 脑部畸形 动物模型 |
Experimental study of mouse infected with human cytomegalovirus AD169 strain] |
| |
| Ni Xie,Li-yu Chen,Gan Dai.Experimental study of mouse infected with human cytomegalovirus AD169 strain][J].Bulletin of Hunan Medical University,2003,28(3):243-246. |
| |
| Authors: | Ni Xie Li-yu Chen Gan Dai |
| |
| Institution: | Department of Microbiology, Xiangya School of Medicine, Central South University, Changsha 410078, China. |
| |
| Abstract: | OBJECTIVE: To determine the sensitivity of mouse to human cytomegalovirus AD169 strain (HCMV AD169) infection and to establish a stable animal model for developmental disorders. METHODS: Forty kunmin mice were divided into two groups randomly: the virus inoculated group and the control group. After 7, 15, 30, and 60 days, the mice's brain development was observed by pathological techniques. HCMV antigen was detected by immunohistochemical method and HCMV DNA was analyzed by polymerase chain reaction (PCR). RESULTS: The extensive pathological damages and developmental disorders in the brain tissues of infected mice were observed. Meanwhile, the viral antigen and viral DNA were also demonstrated in the brain tissues of HCMV infected mice. CONCLUSION: HCMV can cause brain developmental disorders in mice, which provides a rationale for understanding the mechanism of congenital abnormality by HCMV infection. |
| |
| Keywords: | |
| 本文献已被 维普 等数据库收录！ |
