| 氯化锂对HL-60细胞增殖和分化与c-myc表达的影响 |
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| 引用本文： | 李卫民,蒋德昭,卿科云.氯化锂对HL-60细胞增殖和分化与c-myc表达的影响[J].中国实验血液学杂志,1997(2). |
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| 作者姓名： | 李卫民 蒋德昭 卿科云 |
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| 作者单位： | 湖南医科大学血液生理研究室，湖南医科大学血液生理研究室，湖南医科大学血液生理研究室 长沙 410078，长沙 410078，长沙 410078 |
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| 摘 要： | 本实验用细胞培养技术观察了氯化锂对HL-60细胞增殖和分化的影响。不同浓度的氯化锂(5-20mmol/L)对HL-60细胞的液体悬浮培养细胞数以及集落形成和~3H-TdR掺入均呈剂量依赖式抑制;应用NBT还原试验发现氯化锂能诱导HL-60细胞分化。从氯化锂处理的HL-60细胞中提取总RNA,应用RT-PCR检测c-myc mRNA的表达结果表明经氯化锂处理的HL-60细胞c-myc表达,与未处理的HL-60细胞的c-myc比较有明显降低,说明c-myc在白血病细胞增殖、分化中起调控作用。
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| 关 键 词： | 氯化锂 c-myc 原癌基因 HL-60细胞 逆转录-聚合酶链反应 |
Effects of Lithium Chloride on the Proliferation and Differentiation and c-myc Proto-Oncogene Expression in HL-60 Cells |
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| LI Wei-Min JIANG De-Zhao QING Ke-Yun.Effects of Lithium Chloride on the Proliferation and Differentiation and c-myc Proto-Oncogene Expression in HL-60 Cells[J].Journal of Experimental Hematology,1997(2). |
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| Authors: | LI Wei-Min JIANG De-Zhao QING Ke-Yun |
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| Abstract: | This research was to observe the effects of lithium chloride (LiCl) on the proliferation and differentiation of HL-60 leukemia cells. The results obtained by liquid suspension culture, semi-solid colony culture and 3H-TdR incorporation into HL-60 cells indicated that different concentrations of LiCl (5 -20 mmol/L) exerted the inhibitory effects in a dose-dependent manner on HL-60 cell proliferation. It was discovered that there was induction of the differentiation of HL-60 cells by lithium. The results also showed that the expression of the c-myc proto-oncogene in the lithium-treating HL-60 cells was markedly decreased as measured by RT-PCR. These findings provide some evidence of the mechanism of leukemic development and the potential use of lithium in the treatment of leukemia. |
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| Keywords: | lithium chloride c-myc proto-oncogene HL-60 cell RT-PCR |
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