多个单核苷酸多态性位点与食管癌和胃癌患病风险的相关性分析

目的 探讨多个单核苷酸多态性与食管癌和胃癌的关系,寻找在中国河南省汉族人群中食管癌和胃癌的共同遗传风险位点.方法 选择河南省肿瘤医院收治的食管癌患者500例,胃癌患者和健康体检者各600例,挑选了7个单核苷酸多态性(SNP)位点,采用Sequenom MassARRAY SNP基因分型技术进行基因分型.通过无条件逻辑回归计算相对优势比(OR)及其95％的置信区间(95％CI).结果 在等位基因模型下,有2个SNP位点与食管癌的患病风险相关,分别是rs4785204(OR=1.43,95％CI:1.12～1.85,P=0.01)和rs4924935位点(OR=o.76,95％CI:0.61～0.99,P=0.04).有4个SNP位点与胃癌的患病风险相关,分别是rs13361707(OR=1.23,95％CI.1.13～1.56,P=0.00)、rs4779584(OR=1.25,95％CI.1.11～1.58,P=0.04)、rs4785204(OR=1.24,95％CI:1.03～1.49,P=0.03)和rs4924935位点(OR=0.76,95％CI:0.60～0.99,P=0.04).在遗传模型分析中,发现有4个位点与食管癌的患病风险明显相关(P＜0.05),分别是rs6687758、rs401681、rs4785204和rs4924935位点;有3个位点与胃癌的患病风险相关(P＜0.05),分别是rs13361707、rs4779584和rs4785204位点.结论 中国河南省汉族人群中多个SNP位点与食管癌和胃癌的遗传易感性有关联,rs4785204和rs4924935位点的遗传变异可能同时在食管癌和胃癌的发生、发展中起到重要作用.

Analysis on correlation between multiple single nucleotide polymorphisms loci and risk of esophageal and gastric cancer

Objective To investigate the association between multiple single nucleotide polymorphisms (SNP) with esophageal and gastric cancer,and to search the shared genetic risk loci for esophageal and gastric cancer in Han population of Henan Province.Methods Five hundreds cases of esophageal cancer,600 cases of gastric cancer and 600 persons undergoing physical examination in the Henan Provincial Tumor Hospital were selected.Seven SNP loci were selected.The genotyping was performed by adopting the Sequenom MassAR-RAY SNP genoting technique.The relative odds ratios(OR) and 95 ％ confidence intervals(CI) were calculated by unconditional logistic regression.Results In allelic model,the two SNP loci were correlated with the risk of esophageal cancer,which were rs4785204 (OR =1.43,95 ％ CI:1.12-1.85,P =0.01) and rs4924935 (OR =0.76,95 ％CI:0.61-0.99,P=0.04).The four SNP loci were correlated with the risk of gastric cancer,gastric cancer,which were rs13361707(OR=1.23,95％CI:1.13-1.56,P=0.00),rs4779584(OR=1.25,95％CI:1.11-1.58,P=0.04),rs4785204(OR=1.24,95％CI:1.03-1.49,P=0.03)and rs4924935(OR=0.76,95％ CI:0.60-0.99,P =0.04).In the genetic model analysis,the four loci were significantly correlated with the risk of esophageal cancer,which were rs6687758,rs401681,rs4785204 and rs4924935(P＜0.05);the three loci were correlated with the gastric cancer,which were rs13361707,rs4779584 and rs4785204(P＜0.05).Conclusion Multiple SNP loci have the relationship with the risk of esophageal and gastric cancer in Han population of Henan Province.The genetic variation of rs4785204 and rs4924935 loci may simultaneously play an important role in the occurrence and development of esophageal and gastric cancer.