微小RNA-134-5p靶向作用于EGFR对卵巢癌SKOV3和A2780细胞生长的影响

目的 探讨微小RNA-134-5p(miR-134-5p)靶向作用于表皮生长因子受体(EGFR)基因对卵巢癌细胞生长的影响.方法 以卵巢癌细胞系SKOV3和A2780为研究对象,根据处理方法分为对照组(转染miR-NC)和实验组(转染miR-134-5p).采用qRT-PCR和Western blot检测EGFR基因及下游靶蛋白的表达量;流式细胞术检测细胞周期分布和细胞凋亡情况;四甲基偶氮唑蓝(MTT)法和集落形成实验检测卵巢癌细胞增殖能力.结果 实验组EGFR基因及下游靶蛋白表达显著下调,其中SKOV3细胞中EGFR mRNA下调至48％(P＜0.05),A2780细胞中EGFR mRNA下调至47％(P＜0.05).实验组细胞的细胞周期明显受到抑制(P＜0.05),miR-134-5p通过EGFR靶蛋白诱导细胞凋亡(P＜0.05).实验组细胞的增殖活性和集落形成能力明显受到抑制(P＜0.05).结论 miR-134-5p可通过靶向抑制EGFR基因,促进细胞周期停滞和细胞凋亡,降低卵巢癌细胞的增殖能力.

Effect of microRNA-134-5p targeting EGFR on growth of ovarian cancer SKOV3 and A2780 cells

Objective To investigate the effect of microRNA-134-5p (miR-134-5p) targeting epidermal growth factor receptor (EGFR) on the growth of ovarian cancer cells.Methods The ovarian cancer cell lines SKOV3 and A2780 served as the study objects and were divided into the control group (transfecting miR-NC) and experimental group (transfecting miR-134-5p) according to the treatment method.The expression levels of EGFR gene and downstream target protein were detected by qRT-PCR and western blot.The cell cycle distribution and apoptosis were detected by flow cytometry.The proliferation ability of ovarian cancer cells was detected by MTT assay and colony forming assay.Results The expressions of EGFR and downstream target protein in the experimental group were significantly down-regulated.EGFR mRNA in SKOV3 cells was downregulated to 48％ (P＜0.05),and EGFR mRNA in A2780 cells was down-regulated to 47％ (P＜0.05).The cell cycle of cells in the experimental group was significantly inhibited (P＜0.05),and miR-134-5p induced apoptosis through the EGFR target protein (P＜0.05).The proliferation activity and colony forming ability of the experimental group were significantly inhibited (P＜0.05).Conclusion miR-134-5p could promote the cellular cycle arrest and apoptosis,and reduces the proliferation ability of ovarian cancer cells by targetedly inhibiting the EGFR gene.