HIV-1感染者CD73~+CD8~+ T细胞减少与T细胞异常活化的相关性研究

目的探讨HIV-1感染者外周血CD8~+T细胞上CD73的表达特点及其与T细胞异常活化和疾病进展的关系。方法研究入选65例HIV-1感染者和27例健康对照。通过流式细胞术检测研究对象外周血CD73~+CD8~+T细胞的频率和绝对计数,并将患者CD73~+CD8~+T细胞绝对计数和频率与其CD4~+T细胞计数、HIV-1载量以及CD38~+CD8~+T细胞频率进行相关性分析。结果与健康对照相比,HIV-1感染者外周血CD73~+CD8~+T细胞绝对计数和频率均明显降低(P均0.05);HIV-1感染者外周血CD73~+CD8~+T细胞绝对计数和频率与CD4~+T细胞计数呈正相关(r=0.555,P=0.001;r=0.342,P=0.005),与CD38~+CD8~+T细胞频率呈负相关(r=-0.384,P=0.002;r=-0.387,P=0.001);HIV-1感染者CD73~+CD8~+T细胞的绝对计数与HIV-1载量呈弱负相关(r=-0.261,P=0.035)。结论 HIV-1感染者外周血CD73~+CD8~+T细胞的减少不但与患者T细胞的活化程度呈显著负相关,而且与AIDS疾病进展相关。

Correlation between decreased CD73+CD8+T cells and T-cell abnormal activation in HIV-1 infected patients

Objective To detect the expression of CD73 on peripheral blood CD8+T cells in HIV-1 infected patients and analyze its correlation with disease progression and T cell abnormal activation. Methods Twenty-seven health controls and 65 HIV-1 infected patients were enrolled in this study. The frequency and absolute count of peripheral blood CD73+CD8+T cells in all subjects were detected by flow cytometry and its correlation with CD4+T cell counts, HIV-1 viral load and frequency of CD38+CD8+T cells were analyzed. Results Compared with healthy controls, the absolute count and frequency of CD73+CD8+T cells were significantly reduced in peripheral blood of HIV-1 infected patients (P<0.05); the absolute count and frequency of CD73+CD8+T cells in peripheral blood of HIV-1 infected patients were positively correlated with CD4+T cell counts (r=0.555, P=0.001; r=0.342, P=0.005), and negatively correlated with frequency of CD38+CD8+T cells (r=-0.384, P=0.002; r=-0.387, P=0.001); there was a negative correlation between the absolute count of CD73+CD8+T cells and the HIV-1 viral load in HIV-1 infected patients (r=-0.261, P=0.035). Conclusions The decrease of CD73+CD8+T cells in HIV-1 infected patients is not only significantly negatively correlated with T cell activation, but also associated with progression of AIDS.