基于miR-1285在卵巢癌组织中的表达及对癌细胞增殖和凋亡的影响

目的 探索人体卵巢癌细胞微RNA-1285 (miR-1285)的表达及其在卵巢癌病变细胞增殖与凋亡过程中的作用.方法 采用荧光定量PCR法检测卵巢癌患者卵巢癌组织和对应癌旁组织细胞中miR-1285的表达水平;同时在体外进行细胞实验,向人源SKOV3和OVCAR3卵巢癌细胞株转染miR-1285 mimics,运用噻唑兰(MTT)法对转染miR-1285 mimics成功的两株人源卵巢癌细胞进行体外增殖活力的检测,同时通过流式细胞仪检测细胞凋亡的发生情况.结果 荧光定量PCR检测结果表明,miR-1285在患者卵巢癌组织细胞中的表达水平低于其对应癌旁组织,差异有统计学意义(P＜0.05);细胞体外转染miR-1285 mimics实验结果表明,相较于对照组的细胞株,转染miR-1285 mimics的细胞株SKOV3,其细胞增殖能力明显下降(P＜0.05)、细胞凋亡率明显升高(P＜0.05);同样,转染miR-1285 mimics的细胞株OVCAR3其增殖能力也明显下降、细胞凋亡率明显升高,与对照组比较,差异有统计学意义(P＜0.05).结论 在卵巢癌患者的癌细胞中,miR-1285的表达明显降低,其可能参与卵巢癌的发生、发展,并且与卵巢癌细胞的增殖和凋亡密切相关.

Expression of miR-1285 in ovarian cancer tissues and its influence on proliferation and apoptosis of cancer cells

Objective To explore the expression of microRNA-1285 (miR-1285) in ovarian cancer tissues,and to analyse the effects of miR-1285 expression on proliferation and apoptosis of human ovarian cancer cell lines in vitro.Methods The expressions of miR-1285 in cancer tissues and corresponding adjacent tissues from patients with ovarian cancer were detected by using fluorescence quantitative PCR.At the same time,human ovarian cancer cell lines,including SKOV3 and OVCAR3,were transfected with miR-1285 mimics in vitro,the proliferation activity of cells successfully transfected with miR-1285 was detected by MTT assay,and the apoptotic cells were detected by flow cytometry.Results The results of fluorescence quantitative PCR showed that,for patients with ovarian cancer,the expression level of miR-1285 in ovarian cancer tissues was lower than that in the corresponding adjacent tissues,the difference was statistically significant (P＜0.05).The results of experiment in vitro showed that compared with the control group,the proliferation activity of SKOV3 cells transfected with miR-1285 mimics was decreased,and the rate of apoptosis was significantly increased,there were statistically significant differences (P＜0.05);similarly,compared with the control group,the proliferation activity of OVCAR3 cells transfected with miR-1285 mimics was decreased,and the rate of apoptosis was significantly increased,there were statistically significant differences (P＜0.05).Conclusion The expression level of miR-1285 in tumor tissues of patients with ovarian cancer is decreased,which indicates that miR-1285 may be involved in the occurance and progression of ovarian cancer;the experiment in vitro shows that miR-1285 may be closely related to the proliferation and apoptosis of ovarian cancer cells.