广西沿海地区Graves病与HLA-DQA1、DQBIDRB1*09等位基因的相关性研究

目的研究广西沿海地区Graves病（gravesdisease,GD）患者中HLADQA1、DQB1及DRB1＊09等位基因的分布情况及其关系。方法采用序列特异性引物PCR（PCR—SSP）方法检测162例广西沿海地区GD患者及90例健康者的HLA—DQA1、DQB1及DRB1＊09的基因型,计算和比较各组间等位基因频率。结果与健康对照组比较,HLADQA1＊0501、DQB1＊0303和HLA—DRB1＊0901在GD患者组的分布频率明显增高（P〈0．05）;GD女性患者组中的HLADQA1＊0301频率比健康对照组女性明显升高,差异有统计学意义（P〈0．05）。结论HLADQA1＊0501、HLA-DQB1＊0303及HLADRB1＊0901是广西沿海地区GD遗传易感基因;DQA1＊0301是广西沿海地区女性GD患者中的遗传易感基因。

The association between Graves'disease and polymorphism in HLA-DQA1,DQB1 and DRB1*09 alleles in Cuangxi coastal area

Objective To study the distribution of HLA-DQA1,DQB1 and DRB1*09 alleles in patients with Graves'disease(GD)in Guangxi coastal area as well as their association.Methods HLA-DQA1,DQB1 and HLA-DRB1*09 alleles were typed by PCR amplification with sequence-specific primers(PCR-SSP)method in 162 GD patients and 90 normal subjects.The comparison of allele frequencies was made between GD group and healthy control group.Results The gene frequencies of HLA-DQA1*0501,DQB1*0303 and DRB1*0901 were significantly higher in GD group than thoes in healthy control group(P<0.05) In GD group,the frequency of HLA-DQA1*0301 was markedly higher in female subgroup than that in male subgroup(P<0.05).Conclusion HLA-DQA1*0501,DQB1*0303 and DRB1 * 09 are predisposing genes of GD in GuangXi coastal area.HLA-DQA1*0301 allele is the predisposing gene of female GD in the area.