螺旋藻对实验性口腔癌预防作用的研究

目的 研究螺旋藻对二甲基苯并蒽(DMBA)诱发的金黄地鼠口腔癌的预防作用。方法选用DMBA诱发金黄色地鼠口腔癌模型，同时用1.0g/kg、0.3g/kg螺旋藻进行预防，进行口腔肿瘤发生率、癌变率、微核细胞率、细胞增殖核抗原(PCNA)和上皮生长因子受体(EGFR)检测。结果螺旋藻高、低剂量组平均瘤负荷抑制率分别为74.2％和59.8%；癌变率、微核细胞率和PCNA标记指数与阳性对照组相比均明显降低，但对EGFR表达没有影响。结论螺旋藻对DMBA诱发的动物口腔癌有预防作用。

Study of the effects of spirulina on DMBA-induced oral carcinogenesis in hamsters

Objective The preventive effects of spirulina on dimethyl-benzanthracene (DMBA) induced oral carcinogenesis in syrian golden hamster were studied.Methods The hamsters were divided into four groups,the positive control group (the left buccal pouches of animals were topically treated with 0.5% DMBA in acetone three times per week for 14 weeks),two spirulina treated groups (oral administration spirulina 0.3g/kg and 1.0g/kg every day before starting the DMBA treatment and until the end of the expriment) and the negative control group (acetone applied topically).Results Oral admistration of spirulina 0.3g/kg and 1.0g/kg could reduce micronuclei formation and labeling index of proliferating cell nuclea antigen (PCNA) in oral mucosa cells induced by DMBA,but had no effect on the level of epidermal growth factor receptor (EGFR) expression.The average number of tumor per hamster was reduced by 39.8% and 35% respectively,and the mean tumor burden was reduced by 59.8% and 74.2% respectively,compared with the positive control group.Conclusion Spirulina could effectively inhibit DMBA-induced oral carcinogenesis in hamsters.Protecting DNA damage and suppression of cell proliferation are considered as important mechanisms of the anticarcinogenic effects of the spirulina.