|
|||||
|
|
Pharmacological analysis of 125I-Bolton and Hunter labelled eledoisin binding sites in rat spinal cord by quantitative autoradiography |
|
| Authors: | J C Hunter G J Kilpatrick J R Brown |
| Affiliation: | 1. Institute for Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden;2. Center for Radiopharmaceutical Sciences ETH-PSI-USZ, Paul Scherrer Institut, Villigen-PSI, Switzerland;3. Division of Protein Technology, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, Sweden;4. Laboratory of Radiochemistry, Paul Scherrer Institut, Villigen-PSI, Switzerland;5. Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich, Switzerland;1. Laboratory for Radiopharmaceutical Research, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, Leuven, Belgium;2. Centre for Advanced Imaging, University of Queensland, Brisbane, Australia;3. Switch Laboratory, VIB-KU Leuven Center for Brain & Disease Research, KU Leuven, Leuven, Belgium |
| Abstract: | Using the technique of quantitative autoradiography it has been possible to investigate and compare the pharmacological characteristics of 125I-Bolton-Hunter conjugated eledoisin (125I-BHE) binding sites in rat spinal cord with those in the rat cortex. 125I-BHE specific binding sites were discretely localised in the outer layers of the rat spinal cord. The rank order of affinity of unlabelled tachykinins in competing for 125I-BHE specific binding sites in rat spinal cord (NKB greater than ELE greater than NKA greater than SP) was identical to that found in the rat cortex suggesting the presence of 'NK-3 like' receptors in rat dorsal horn. |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ | |