复合胶原的组织工程肌腱力学性能的实验研究

目的探讨胶原在组织工程肌腱的构建中对力学强度的影响. 方法裸鼠75只,制成跟腱缺损的动物模型,随机分为5组,分别将人发(hair,H)、碳纤维(carbon fiber,CF)、聚羟基乙酸(polyglycolic acid,PGA)、H PGA和CF PGA复合外源性Ⅰ型胶原,与标准转化人胚腱细胞株联合培养制成组织工程肌腱.植入裸鼠右后肢修复跟腱缺损,为实验组(A组);将未复合胶原的上述各种材料植入裸鼠左后肢修复跟腱缺损,为对照组(B组).术后于2、4、6、8和12周进行生物力学测定,观察并比较其变化. 结果实验组2～4周力学强度高于对照组,差异有统计学意义(P＜0.05),但6～12周两组差异无统计学意义(P＞0.05);时间与力学强度呈正相关,差异有统计学意义(P＜0.05).其中以人发的力学强度最佳,CF次之,PGA最差. 结论外源性胶原在肌腱修复的早期能增强组织工程肌腱的力学强度,对患肢功能的恢复有一定的作用.

EXPERIMENTAL STUDY ON BIOMECHANICS CHARACTERISTICS OF COMBINED COLLAGEN TISSUE ENGINEERING TENDON

Objective To investigate the influence of collagen on the biomechanics strength of tissue engineering tendon. Methods All of 75 nude mice were made the defect models of calcaneous tendons, and were divided into 5 groups randomly. Five different materials including human hair, carbon fibre (CF), polyglycolic acid (PGA), human hair and PGA, and CF and PGA with exogenous collagen were co-cultured with exogenous tenocytes to construct the tissue engineering tendons. These tendons were implanted to repair defect of calcaneous tendons of right hind limb in nude mice as experimental groups, while the materials without collagen were implanted to repair the contralateral calcaneous tendons as control groups. In the 2nd, 4th, 6th, 8th and 12th weeks after implantation, the biomechanical characteristics of the tissue engineering tendon was measured, meanwhile, the changes of the biomechanics strength were observed and compared. Results From the 2nd week to the 4th week after implantation, the experimental groups were stronger than the control groups in biomechanics, there was statistically significant difference ( P <0.05). From the 6th to 12th weeks, there was no statistically significant difference between the experiment and control groups ( P >0.05). Positive correlation existed between time and intensity, there was statistically significant difference ( P <0.05). The strength of materials was good in human hair, followed by CF, and PGA was poor. Conclusion Exogenous collagen can enhance the mechanics strength of tissue engineering tendon, and is of a certain effect on affected limb rehabilitation in early repair stages.