十溴联苯醚-209对原代培养的胎鼠海马神经细胞中超氧化物歧化酶、丙二醛及谷胱甘肽的影响

目的 检测十溴联苯醚-209(BDE-209)对原代培养胎鼠海马神经元细胞中超氧化物歧化酶(SOD)活力、丙二醛(MDA)及谷胱甘肽(GSH)含量的影响,探讨BDE-209对海马神经元细胞影响的作用机制.方法 将原代培养的胎鼠海马细胞分为A、B、C、D、E组,分别暴露于2.5、5.0、10.0、20.0、40.0μg/ml BDE-209,同时设一组对照组,BDE-209暴露剂量为0.每组3个平行样,24 h后检测SOD活力、MDA及GSH含量.结果 SOD活力随染毒剂量增加而降低,实验A～E组(29.809±3.269;28.424±1.953;26.912±1.439;25.533±2.259;15.678±2.607)与对照组(38.056±4.210)比较,差异均有统计学意义(P<0.01).各实验组MDA含量随染毒剂量增加而增加,其中C组(1.421±0.531)、D组(1.997±0.499)、E组(2.233±0.865)与对照组(0.544±0.236)比较,差异有统计学意义(P<0.01).实验A～E组GSH含量随染毒剂量增加而减少(288.576±77.514;257.929±70.959;126.349±40.649;76.350±13.850;59.634±17.533),与对照组(347.228±38.162)比较,差异均有统计学意义(P<0.05).结论 BDE-209暴露原代培养胎鼠海马神经元细胞可发生氧化损伤,这可能是BDE-209神经毒性的作用机制之一.

Effect of brominated diphenyl ethers-209 on superoxide dismutase,malondialdehyde and glutathione reductase in primary cultured hippocampal neurons of fetal rats

Objective To determine the effect of brominated diphenyl ethers-209 (BDE-209) on superoxide dismutase (SOD) activity,malondialdehyde (MDA) and glutathione reductase (GSH) concentrations in the hippocampal neurons of fetal rats.Methods The activities of SOD,concentrations of MDA and GSH were measured respectively after the rat hippocampal neurons were exposed to 2.5 (group A),5.0(group B),10.0(group C),20.0(group D),40.0 μg/ml(group E) BDE-209 for 24 h in vitro.Those rats without BDE-209 exposure were served as control.Results The SOD activity in the hippocampus was decreased with the increased exposure to BDE-209,and the experimental groups showed significant difference from the control group(29.809±3.269,28.424±1.953,26.912±1.439,25.533±2.259,15.678±2.607 vs 38.056±4.210,P<0.01);The MDA contents in the five experimental groups also increased with increase of BDE-209 exposure,and that in group C(1.421±0.531),D(1.997±0.499),and E(2.233±0.865) was higher than in the control group (0.544±0.236),respectively (P<0.01).However,the GSH contents was decreased with the increased exposure to BDE-209,and that in each experiment group was significantly lower than in the control (288.576±77.514,257.929±70.959,126.349±40.649,76.350±13.850,59.634±17.533 vs 347.228±38.162,P<0.05).Conclusions BDE-209 exposure can induce damage of nervous system during fetal development through accelerating lipid peroxidation of cerebral tissues.