基于GC-MS的金欣口服液对RSV肺炎小鼠脾脏代谢物的调控作用

目的通过气相色谱-质谱联用(GC-MS)技术分析呼吸道合胞病毒(RSV)感染小鼠脾脏组织中内源性代谢物的变化,探讨金欣口服液治疗RSV肺炎可能的作用机制。方法 RSV滴鼻感染BALB/c小鼠建立RSV肺炎模型,给予金欣口服液(27.6 g/kg)干预治疗,连续治疗7 d后处死小鼠,取脾脏组织,经甲醇提取后,先后予甲氧胺吡啶和BSTFA以肟化和衍生化,然后运用GC-MS检测脾脏中所含有的代谢物及其相对量的变化。结果 RSV肺炎小鼠的脾脏中共鉴定出35种代谢产物,其中L-脯氨酸、L-谷氨酸、缬氨酸、尿素、次黄嘌呤、葡萄糖为具有显著性差异的代谢物(P0.05),与之相关的代谢通路为D-谷氨酰胺和谷氨酸代谢,缬氨酸、亮氨酸和异亮氨酸代谢,丙氨酸、天冬氨酸和谷氨酸代谢,精氨酸和脯氨酸代谢。结论金欣口服液可以调节RSV肺炎小鼠脾脏的免疫功能,可能与氨基酸代谢有关。

Regulation of Jinxin Oral Liquid on metabolites in spleen of mice with RSV pneumonia based on GC-MS

Objective To detect the relative abundance changes of metabolites in spleen to explore the antiviral effect of Jinxin Oral Liquid (JOL) against RSV pneumonia by GC-MS technology. Methods BALB/c mice were challenged intranasally to establish the RSV pneumonia models and ig administered with JOL (27.6 g·kg^-1·d^-1). After treated for 7 d, mice spleens were collected respectively. Metabolites were extracted by methanol, and then oximated, derevatized, and detected by GC-MS. Results 35 metabolites in spleen were identified, among which L-proline, L-glutamic acid, valine, urea, hypoxanthine, glucose were statistically significant (P<0.05). Conclusion Anti-RSV infection of JOL may be associated closely with the regulation of immune function of mice spleen, and amino acid metabolism is involved.