MMP-9和TIMP-1在膀胱癌中的表达及临床意义

目的：研究基质金属蛋白酶9（MMP-9）和组织型金属基质蛋白酶抑制因子1（TIMP-1）在膀胱移行细胞癌中的表达及其与肿瘤侵袭转移的关系。方法：采用免疫组织化学SP法检测56例膀胱移行细胞癌标本中MMP-9和TIMP-1的表达，并与肿瘤的临床及病理参数相比较。结果：在56例膀胱移行细胞癌中，MMP-9和TIMP-1的表达率分别为6．1．29％和57．14％，MMP-9表达率随肿瘤的临床分期、病理分级的升高而增加，TIMP—1表达率也呈上升趋势，两者间的表达仔在轻度正相关。结论：MMP-9和TIMP—1的相互作用对于膀胱移行细胞癌的浸润转移发挥了重要作用，MMP-9可能成为早期无创诊断膀胱肿瘤的生物学指标。

Expression of MMP-9/TIMP-1 in bladder and the clinical significance transitional cell carcinoma

Objective:To explore the expression of matrix metalloproteinase-9(MMP-9) and tissue inhibitor of metalloproteinase-1(TIMP-1) in bladder cancer and their clinical significance.Methods:The immunohistochemical staining assay was conducted to detect expression of MMP-9/TIMP-1 in 56 cases of bladder transitional cell carcinoma. The relationships among MMP-9/TIMP-1 expression, pathological grades and clinical stages of BTCC were also analyzed.Results:The positive rates of MMP-9 and TIMP-1 in bladder cancer are (64.29)%?(57.14)% respectively. MMP-9 expression increased significantly as the clinical stages and pathological grades. With the improvement of invasion tendency of BTCC, the expression of TIMP-1 was gradually increased. The two biomarkers have low positive correlation.Conclusions:The interaction between MMP-9 and TIMP-1 may play an important role in the invasive and metastatic progression of BTCC. Moreover, MMP-9 is regarded as a prognostic indicator of clinical early stage.