整合素αV对鼻咽癌CNE-2Z细胞放疗敏感性的影响

目的：探讨粘附分子整合素αV通过细胞间相互作用，是否对鼻咽癌CNE-2Z细胞的放疗敏感性产生影响，进而初步探讨其可能机制。方法：采用liquid overlay技术进行CNE-2Z细胞三维立体（多细胞球）培养；血球计数器计数法比较在阻断整合素αV作用前后CNE-2Z细胞的放射敏感性；AnnexinV／PI双标流式细胞术检测细胞凋亡。结果：（1）成功建立CNE-2Z多细胞球培养模型；（2）CNE-2Z多细胞球在接受X射线照射后，并在一定的照射剂量下，其表达的整合素αV量，随着照射剂量的增加而增加；（3）阻断整合素αV作用后CNE-2Z多细胞球放疗敏感性增强、细胞凋亡率上调。结论：以多细胞球培养模型模拟体内实体瘤情况，证实粘附分子整合素αV的表达情况可以影响鼻咽癌CNE-2Z细胞的放疗敏感性，抑制细胞凋亡是其可能的作用机制。

Role of integrin alpha V in sensitivity to radiotherapy of human NPC

Objective：To investigate whether the cell adhesion molecule integrin alpha V could influence the radiosensitivity of human nasopharyngeal carcinoma CNE-2Z cells via effecting intercellular effect. Methods：Human NPC cell line CNE-2Z were cultivated as three dimensional model using liquid overlay technique. Cell counting using blood cell counter was employed to detect the radiosensitivity of CNE-2Z cells before and after blocking integrin alpha V function; Cell apoptosis was detected by flow cytometry using Annexin V/PI label. Results：Three dimensional culture model of human NPC cell line CNE-2Z cells were successfully established. The integrin alpha V expression of CNE-2Z muhicellular spheroids elevated as the dosage of radiotherapy increased. Blocking of integrin alpha V function lead to higher radiosensitivity and more apoptotic cells of CNE-2Z muhicellular spheroids. Conclusion：Using three dimensional culture model to mimic solid tumor in vivo, it was proved that the cell adhesion molecules integfin alpha V could influence the radiosensitivity of human nasopharyngeal carcinoma CNE-2Z cells and inhibition of cell apoptosis might be its mechanism.