Improvement of Bone Quality in Gonad-Intact Middle-Aged Male Rats by Long-Chain n-3 Polyunsaturated Fatty Acid |
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Authors: | C-L Shen J K Yeh J Rasty M-C Chyu D M Dunn Y Li B A Watkins |
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Institution: | (1) Department of Pathology, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA;(2) Bone Metabolism Laboratory, Winthrop-University Hospital, Mineola, NY 11501, USA;(3) Department of Mechanical Engineering, Texas Tech University, Lubbock, TX 79401, USA;(4) Center for Enhancing Foods to Protect Health, Lipid Chemistry and Molecular Biology Laboratory, Purdue University, West Lafayette, IN 47907, USA |
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Abstract: | The effect of long-chain n-3 polyunsaturated fatty acid (PUFA) on bone measurements was evaluated in gonad-intact middle-aged
male rats. Seven rats were killed on day 0 of dietary intervention to determine bone parameters at baseline. Experimental
rats (7/group) were fed one of the following lipid treatments (g/kg diet): 167 g safflower oil + 33 g menhaden oil (N6+N3
diet, control), 200 g safflower oil (N6 diet), or 190 menhaden oil + 10 g corn oil (N3 diet). After 20 weeks of dietary treatment,
all groups had lower values for peak load and ultimate stiffness in femurs compared to baseline values. Rats fed the N3 diet
had the highest values for peak load, ultimate stiffness, and Young’s modulus compared with those fed the N6 and control diets.
Compared to baseline, all dietary treatment groups had significantly lower values for trabecular thickness and number in proximal
tibia but higher values for trabecular separation and formation rate in proximal tibia and endocortical bone formation rate
in tibial shaft. Compared with the control group, rats fed the N3 diet had lower values for formation rate, osteoclast number,
and eroded surface in proximal tibia but higher values for periosteal mineral apposition and formation rates in tibia shaft.
These findings indicate that a diet rich in long-chain n-3 PUFA mitigate aging-induced loss of bone integrity in intact middle-aged
male rats through reducing bone turnover rate by suppressing both bone formation and resorption as a result of a larger net
bone volume and modulating endocortical and cancellous bone compartments.
Previously presented in part at the 26 th annual meeting of the American Society of Bone and Mineral Research, Seattle, Washington,
USA, September 2004, and published in abstract form (Shen CL, Dunn DM, Yeh JK 2005] Dietary fish oil mitigates aging-induced
bone loss in middle-aged male rats abstract]. J Bone Miner Res 19(suppl 1):S205). |
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Keywords: | Menhaden oil Histomorphometry Bone mechanical strength Male rat Aging |
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