大鼠腹腔感染致早期肺损伤的实验研究

探讨肠源性感染致早期肺损伤的作用机理。方法，采用大鼠盲肠结扎并并穿民腹腔感染。检测肺毛细管管通透性，取支气管肺泡灌洗液进行细胞学分析，检测血浆，肺组织和ＢＡＬＦ的内毒素和肿瘤坏死因子。结果肺毛细血管通透和ＢＡＬＦ的中性粒细胞百分率逐渐增加，时间越长越明显。血浆、肺组织和ＢＡＬＦ的内毒素逐渐增加，三者之间两两显著相关；ＴＮＦ也逐渐增加，肺组织和ＢＡＬＦ的ＴＮＦ显著相关，两都是民血浆的ＴＮＦ无明显相关

Experimental study of early lung injury caused by abdominal infection in rats

Objective To study the mechanism of early lung injury caused by intestinal infection in rats.Methods The cecal ligation and perforation(CLP) was utilized to make the abdominal infection in rats.The pulmonary vascular permeability and the differential cell count in bronchoalveolar lavage fluid(BALF) were examined. The concentrations of endotoxin and tumor necrosis factor(TNF) in plasma, lung and BALF were detected.Results The pulmonary vascular permeability and the neutrophil percentage of BALF increased progressively. The concentrations of endotoxin and TNF in plasma, lung and BALF significantly increased. The significant correlation was respectively present between the endotoxic levels of plasma, lung and BALF and between the TNF levels of lung and BALF, but not between the TNF level of plasma and one of lung or BALF.Conclusion The abdominal infection might lead to increase of endotoxin, accummulation of neutrophils and release of TNF in lung, which were contributed to the inflammatory reaction of lung. [