高效液相色谱法和化学发光酶免疫法测定万古霉素的血药浓度

目的采用高效液相色谱法和化学发光酶免疫法测定万古霉素血药浓度，比较两种方法测定万古霉素血药浓度的差异。方法取30例静脉滴注万古霉素的患者用药前后的血清样本，应用高效液相色谱法和化学发光酶免疫法测定血药浓度，并分析两种方法测定值的相关性。结果高效液相色谱法在5．0～80．0μg／mL质量浓度内线性关系良好，回收率为97．70％～103．00％，日内、日间RSD分别为4．94％-5．83％和4．53％～5．75％；化学发光酶免疫法的线性范围在5．0～100．0μg／mL，回收率为98．72％～102．70％，日内、日间RSD分别为4．48％～5．62％和6．48％～7．96％。两法测定值相关性良好，r=0．9871。结论两法测定结果并不需要相互校正，临床治疗药物监测测工作中可以根据监测要求和监测目的灵活运用。

Determination of Vancomycin Concentration in Human Plasma by High Performance Liquid Chromatography and Chemiluminescence Enzyme Immunoassay

Objective To determine the vancomycin concentrations in human plasma by the high performance liquid chromatography （HPLC） and the chemiluminescence enzyme immnnoassay （CLEIA） and to compare the difference between the two methods. Methods The serum specimens before and after medication collected from 30 patients with vancomycin by intravenous infusion were detected the plasma drug concentration by HPLC and CLEIA. The relativity between the values detected by these two methods was analyzed. Results The HPLC method had the good linear relationship within the mass concentration range of 5.0- 80.0 μg/mL, and the recovery rate was 97.70%- 103.00%. Intra-day and inter-day RSD were 4. 94%-5.83% and 4.53%-5.75%. The CLEIA method had the linear relationship within the mass concentration range of 5.0- 100.0 μg/mL,and the recovery rate was 98.72% -102.70%. Intra- day and inter-day RSD were 4.48% -5.62% and 6.48% -7.96%. The detected values by the two methods had a good correlation （r = 0, 987 1 ）. Conclusion The determination results by the two methods do not need the mutual correction. In the clinical surveillance works of the therapeutical drugs,these two methods may be flexibly applied according to the monitoring requirements and purpose.