地塞米松、FK-506和CX-659s对TARC产生的调控作用研究

目的研究地塞米松、他克莫司（FK-506）和CX-659s对胸腺活化调节趋化因子（TARC／CCL17）产生的调控作用。方法运用ELISA方法分别研究了3种药物对人角质形成细胞株——HaCd细胞和人成纤维细胞株——NG1RGB细胞的TARC表达作用的影响。结果3种药物对于HaCaT细胞中TARC的表达，10^-9～10^-5mol／L地塞米松显示明显的抑制作用；对于IFN-γ刺激下HaCaT细胞产生TARC水平，10^-12～10^-6mol／L地塞米松、10^-12～10^-6mol／L的FK-506均表现为明显的抑制作用（P〈0．05或P〈0．01）；CX-659s显示明显抑制NGlRGB细胞中TARC水平，且具有浓度依赖性（P〈0．01）。3种免疫抑制剂分别在不同条件下抑制皮肤角质形成细胞和／或成纤维细胞的TARC表达。结论这些药物明显抑制TARC在皮肤角质形成细胞和成纤维细胞的表达，因此认为角质形成细胞和成纤维细胞可能在病理状态下通过促进CCR4＋T细胞向炎症部位的游走，参与免疫调控作用。

Regulatory Effect of Dexamethasone,Fk- 506 and CX- 659s on TARC

Objective To study the regulatory effect generated by dexamethasone,tacrolimus （FK506） and CX- 659s on thymus activa- tion regulated ehemokine （TARC/CCL17）. Methods The ELISA method was adopted to study the influence of these 3 kinds of drug on the TARC expression role of human keratinocytes cell line- HaCaT cell and fibroblasts eel1 line- NG1RGB cell. Results For TARC expression of HaCaT cell,10^-9 -10^- 5 mol/L dexamethasone displayed the obviously inhibiting effect;for HaCaT producing TARC level under IFN-γ stimulation,10^-2- 10^-6 mol/L dexamethasone and 10^-12- 10^-6 mol/L FK-506 all revealed the obvious inhibiting role （P 〈 0. 05 or P 〈 0.01）;CX-659s demonstrated the obvious inhibition on TARC level in NG1RGB with concentration dependency （P 〈 0.01）. 3 kinds of immunosuppressive agents inhibited the TARC expression of keratinocytes and/or fibroblasts under different conditions. Conclusion These immunosuppressive drugs obviously suppress the TARC expression in keratinocytes and fibrob- lasts. Therefore, it is suggested that both keratinocytes and fibroblasts might participate in the immunoregulation role by promoting CCR4＋T cells migration to the inflammatory position under the pathological condition.