慢性乙型肝炎患者体内干扰素产生细胞的初步检测及其临床意义

目的　研究慢性乙型肝炎患者体内干扰素产生细胞 (IPCs)的特点。方法　用流式细胞仪对 2 1例慢性乙型肝炎患者和 12名健康人外周血中IPCs数量的变化进行了检测 ,同时与T淋巴细胞亚群CD4 /CD8 的比值进行了比较分析。结果　慢性乙型肝炎患者外周血IPCs的百分数 ( 0 .0 97± 0 .0 82 )较正常人 ( 0 .310± 0 .0 87)明显降低 ,两者相差3.2倍 ,有显著差异 (P <0 .0 0 1) ,而CD4 /CD8 的比值 ( 1.79± 0 .46 1)则明显高于正常人的 1.39± 0 .396 (P <0 .0 1) ,同时发现在HBVDNA阳性的患者中 ,其IPCs的百分数和CD4 /CD8 的比值分别为 0 .0 83± 0 .0 5 7和 1.98± 0 .5 2 7,均明显高于HBVDNA阴性患者 (分别为 0 .0 42± 0 .0 2 9和 1.47± 0 .46 6 ) ,差异显著 (P <0 .0 1)。结论　与正常人相比 ,慢性乙型肝炎患者外周血中IPCs的数量降低 ,后者可能与慢性乙型肝炎患者病程的进展以及血清中HBVDNA阳性有关 ,确切机制有待进一步研究。

Characterization of professional Interferon-producing cells in patients with chronic hepatitis B

Objective To investigate the characteristics of professional interferon producing cells (IPCs) from peripheral blood of patients with chronic hepatitis B. Methods Used flow cytometry to quantify the IPCs in twenty one chronic patients (treated group) and twelve healthy individuals (control group) and to analyze the CD4 /CD8 ratio in the two groups.Results The percentage of IPCs from the peripheral blood of chronic hepatitis B patients was significantly lower than that of normal controls ( P <0.001) while the CD4 /CD8 ratio was higher than that of the normal controls ( P <0.01). The percentage of IPCs and CD4 /CD8 ratio were higher in the patients with positive sera HBV DNA, than those with negative sera HBV DNA( P <0.01).Conclusion The decrease of IPCs level from peripheral blood of patients with chronic hepatitis B may be related to the advancement of the disease and the positive sera HBV DNA. Their precise mechanisms remain to be clarified.