基于蛋白酶激活受体2表达研究药对防风-乌梅抗过敏的配伍机制

目的观察防风和乌梅配伍前后对肥大细胞脱颗粒以及蛋白酶激活受体2(PAR-2)表达的影响,为药对防风-乌梅抗过敏的研究提供实验依据。方法体外采用胰蛋白酶刺激小鼠肥大细胞株P815方法建立肥大细胞脱颗粒模型,运用RT-PCR、Western blotting、ELISA等技术,观察防风-乌梅配伍前后对肥大细胞分泌组胺、PAR-2 mRNA和蛋白表达的影响。结果防风-乌梅配伍后在体外抑制肥大细胞分泌组胺、PAR-2表达的作用明显增强,与细胞模型组比较有统计学差异(P0.05或P0.01)。结论防风-乌梅配伍后抑制脱颗粒作用明显增强,下调肥大细胞PAR-2表达可能是药对防风-乌梅抗过敏的配伍机制之一。

Anti-allergy mechanism of fangfeng-wumei pair based on expressions of PAR-2

Objective By observing the effects fangfeng-wumei pair on degranulation of mast cells and PAR-2 expression before and after the pairing to provide experimental basis for the research on its anti-allergy mechanism.Methods A mast cell degranulation mouse model was established by stimulating mouse mast cell line P815 with trysin in vitro, and the levels of histamine as well as PAR-2mRNA and protein expression of P815 cells affected by fangfeng-wumei pair were measured using RT-PCR, Western blotting and ELISA.Results Fangfeng-wumei pair in vitro significantly inhibited the secretion of histamine and the expression of PAR-2 by mast cells in comparison to cell model group ( P <0.05 or P<0.01 ) .Conclusion The results demonstrated that inhibition of degranulation of mast cells was significantly enhanced by fangfeng-wumei pair, and that the expression of PAR-2 by mast cells might be involved in the anti-allergy mechanism of fangfeng-wumei pair.